The rising consumer demand for alternative and sustainable protein sources drives the popularity of the use of plant-based proteins in the pet food industry. Pulse crops, which include beans, peas, lentils, and chickpeas, have become an important addition to both human and animal diets due to their protein content and functional properties. However, knowledge of their nutrient composition and protein quality is necessary for the proper formulation of these ingredients in pet foods. The objective of this study was to determine the macronutrient composition and standardized amino acid digestibility and to describe the protein quality through the use of digestible indispensable amino acid scores (DIAAS-like) of five pulse ingredients. Black bean (BB) grits, garbanzo beans (GB), green lentils (GL), navy bean (NB) powder, and yellow peas (YP) were analyzed for dry matter (DM), ash and organic matter (OM), crude protein (CP), gross energy (GE), acid hydrolyzed fat (AHF), and total dietary fiber (TDF) to determine the macronutrient composition. Precision-fed rooster assays were conducted using cecectomized roosters to calculate standardized amino acid digestibility and true metabolizable energy corrected for nitrogen (TMEn). The essential amino acids, with the exception of methionine, were highly digestible with digestibility values of 80% to 90% (dry matter basis) for all selected pulse ingredients. BB grits had the lowest (P < 0.05) digestibility of arginine (86.5%) and histidine (80.6%) in contrast to GB (94.9% and 89.9%, respectively). The TMEn of GB was highest (P < 0.05) at 3.56 kcal/g compared with the other pulses. The DIAAS-like values for adult dogs were consistently the lowest for methionine for all pulses, making it the first-limiting amino acid in these ingredients. The DIAAS-like values for adult cats showed GL had lowest (P < 0.05) score in tryptophan compared with other pulses when using both AAFCO values and NRC recommended allowances as reference proteins. Methionine was the first-limiting amino acid for YP and tryptophan for GL. Based on macronutrient composition, protein quality, and amino acid digestibility, it can be concluded that pulse ingredients have the required nutritional characteristics to be viable protein sources in canine and feline foods. However, the use of complementary protein sources is recommended to counterbalance any potential limiting amino acids in pulse ingredients.

中文翻译：

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常量营养成分，真正的代谢能和氨基酸消化率，以及用于犬类和猫类饮食的豆类必不可少的氨基酸评分。

消费者对替代和可持续蛋白质来源的需求不断增长，推动了在宠物食品行业中使用基于植物的蛋白质的普及。由于豆类的蛋白质含量和功能特性，其中包括豆类，豌豆，小扁豆和鹰嘴豆的豆类作物已成为人类和动物饮食的重要补充。但是，要正确地在宠物食品中配制这些成分，必须了解其营养成分和蛋白质质量。这项研究的目的是确定常量营养成分和标准化的氨基酸消化率，并通过使用五个脉冲成分的可消化的不可缺少的氨基酸评分（类DIAAS）来描述蛋白质质量。黑豆（BB）沙粒，鹰嘴豆（分析了GB，绿扁豆（GL），海军豆（NB）粉和黄豌豆（YP）的干物质（DM），灰分和有机物质（OM），粗蛋白（CP），总能量（GE） ，酸水解脂肪（AHF）和总膳食纤维（TDF）来确定常量营养成分。使用切除后的公鸡进行精确喂养的公鸡测定，以计算标准化的氨基酸消化率和经氮校正的真实代谢能（TMEn）。除蛋氨酸外，必需氨基酸具有很高的消化率，所有选定豆类成分的消化率均为80％至90％（以干物质计）。与GB（分别为94.9％和89.9％）相比，BB粗粉对精氨酸（86.5％）和组氨酸（80.6％）的消化率最低（P <0.05）。与其他脉冲相比，GB的TMEn在3.56 kcal / g时最高（P <0.05）。成年犬的DIAAS样值始终是所有豆类中蛋氨酸的最低值，使其成为这些成分中的第一个限制性氨基酸。成年猫的DIAAS样值显示GL最低（P当同时使用AAFCO值和NRC推荐的允许量作为参考蛋白时，色氨酸与其他脉冲相比得分<0.05）。蛋氨酸是YP的第一个限制性氨基酸，而色氨酸是GL的第一个限制性氨基酸。根据大量营养成分，蛋白质质量和氨基酸消化率，可以得出结论，豆类成分具有必需的营养特性，才能成为犬类和猫类食品中可行的蛋白质来源。但是，建议使用补充蛋白质源来抵消豆类成分中任何潜在的限制性氨基酸。