ABSTRACT

This study aimed to enhance the stability and compatibility of swertiamarin by loading it on amino-functionalised graphene oxide (GO) by ultrasonication. The nanoformulation showed a new peak in the FTIR spectrum (1658 cm⁻¹) and a coupled peak in the UV-Vis spectrum implying CO-NH₂ interaction between swertiamarin and functionalised GO. The SEM image revealed a sheet-like appearance with an average particle size of 252.38 ± 86.09 nm. The nanoformulation was non-haemolytic at 200 μg/mL (<5%) whereas swertiamarin was haemolytic at 50 μg/mL concentration. The freeze-dried samples exhibited good stability at normal storage condition. The formulation and the swertiamarin displayed similar anticancer activity against HepG2 cell lines with an average IC₅₀ value of 110 ± 0.75 μg/mL, respectively. Hence, the functionalised GO can be considered as a compatible carrier for bioactive compounds which provides better hemocompatibility and stability.

摘要

本研究旨在通过超声处理将其负载在氨基功能化的氧化石墨烯 (GO) 上来提高 swertiamarin 的稳定性和相容性。纳米制剂在 FTIR 光谱 (1658 cm ^-1 ) 中显示出一个新峰，并在 UV-Vis 光谱中显示出一个耦合峰，这表明药草甙和功能化 GO 之间的CO-NH ₂相互作用。SEM 图像显示平均粒径为 252.38 ± 86.09 nm 的片状外观。纳米制剂在 200 μg/mL (<5%) 时是非溶血性的，而 50 μg/mL 浓度下的药香素是溶血性的。冻干样品在正常储存条件下表现出良好的稳定性。该制剂和 swertiamarin 对 HepG2 细胞系显示出相似的抗癌活性，平均 IC ₅₀值分别为 110 ± 0.75 μg/mL。因此，功能化的 GO 可以被认为是生物活性化合物的相容载体，提供更好的血液相容性和稳定性。