ABSTRACT

Here, human genomic DNA (hDNA) was encapsulated into supermacroporous alginate beads (SMPA) fabricated via microinjection and freeze-thawing cryogelation protocol. The scanning electron microscopy of the SMPA beads demonstrated smooth and centred burgled morphologies, while the encapsulated hDNA showed brilliant green fluorescence when visualised by fluorescence microscopy. The encapsulation efficiency of 89.1 − 96.7% was achieved when the concentration of hDNA and alginate varied within 0.05 − 0.075% and 0.5 − 0.75 wt%, respectively in the presence of 0.1 M CaCl₂ crosslinking agent. ~80% hDNA was released over an extended period of 80 h when SMPA was immersed in a 0.5 M Na₂HPO solution diluted with 10 mM Tris buffer (pH 7). Results and trends here demonstrated that SMPA beads have great potential to be used as a biocompatible vehicle for transporting biomacromolecules.

摘要

在这里，人类基因组DNA（h DNA）被封装到通过显微注射和冻融冷冻凝胶协议制造的超巨藻藻酸盐珠（SMPA）中。SMPA珠的扫描电子显微镜显示出光滑且居中的盗贼形态，而通过荧光显微镜观察时，包封的h DNA显示出亮绿色的荧光。当在0.1 M CaCl ₂交联剂存在下，h DNA和藻酸盐的浓度分别在0.05-0.075％和0.5-0.75 wt％之间变化时，包封效率达到了89.1 – 96.7％。将SMPA浸入0.5 M Na _2中时，在80 h的较长时间内释放了约80％h的DNA用10 mM Tris缓冲液（pH 7）稀释的HPO溶液。此处的结果和趋势表明，SMPA磁珠具有巨大的潜力，可以用作生物相容性载体来运输生物大分子。