Abstract Lipases are one of the most important catalysts for several industries such as detergent, dairy, and textile industry due to their bio-catalytic ability in aqueous and non-aqueous media. Stability to extreme conditions is an important property since it makes enzymes suitable to several industrial processes. In this study, lipase producing soil bacteria were screened and identified with 16S rDNA sequencing. A new hyper-thermophilic lipase named as Bacillus subtilis LP2 isolate was partially purified by ammonium sulphate precipitation with 17.8-fold purification and 583 U/mg specific activity. Maximum activity was exhibited at pH 7 and 80 °C with the substrate tween 80 KM and Vmax values were calculated as 18.3 mM and 680 U/mg with a catalytic efficiency (kcat/KM) of 307 s−1M−1. These results indicate that lipase from Bacillus subtilis LP2 can be a valuable candidate for industrial applications such as organic synthesis and fats and oils industry due to their efficient catalysis in higher temperatures.

中文翻译：

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枯草芽孢杆菌 LP2 新分离株产生的超嗜热脂肪酶的筛选、部分纯化和表征

摘要 脂肪酶因其在水性和非水性介质中的生物催化能力而成为洗涤剂、乳制品和纺织工业等多个行业最重要的催化剂之一。极端条件下的稳定性是一个重要特性，因为它使酶适用于多种工业过程。在这项研究中，通过 16S rDNA 测序筛选和鉴定了产生脂肪酶的土壤细菌。一种名为枯草芽孢杆菌 LP2 分离株的新型超嗜热脂肪酶通过硫酸铵沉淀法进行了部分纯化，纯度为 17.8 倍，比活性为 583 U/mg。在 pH 7 和 80 °C 下表现出最大活性，底物为 80 KM，Vmax 值计算为 18.3 mM 和 680 U/mg，催化效率 (kcat/KM) 为 307 s-1M-1。