Th2 immune cells infiltration into nasal mucosa is one of the characters of allergic rhinitis (AR). We aimed to explore whether inhibition of Th2 immune cells infiltration would attenuate AR progression. AR mouse model was established by i.p. injection of ovalbumin (OVA). The infiltrated immune cells into nasal lavage fluid were detected by flow cytometry. Cytokine concentration in serum was determined by ELISA. AR mice symptoms were indicated by the number of sneezing and nasal rubbing events. In AR mice, CCL2 expression levels and CD45⁺CD11b⁺Ly6C^hi inflammatory monocytes cells significantly increased as compared with control mice. CCL2 siRNA encapsulated nanoparticles (NP_siCCL2) prevent CCL2 expression and inflammatory monocytes infiltration in AR mice. NP_siCCL2 treatment dramatically decreased the number of sneezing and nasal rubbing events in AR mice. Moreover, NP_siCCL2 treatment attenuated serum OVA-specific IgE, OVA-specific IgG1 and histamine levels. Mechanically, NP_siCCL2 treatment attenuates AR symptoms via inhibiting Th2 cytokine (IL-4, IL-5 and IL-13) production. Nanomedicine-mediated prevention of inflammatory monocytes infiltration ameliorates ovalbumin-induced allergic rhinitis in mouse model.

Th2免疫细胞浸入鼻粘膜是变应性鼻炎（AR）的特征之一。我们旨在探讨抑制Th2免疫细胞浸润是否会减弱AR进展。通过腹腔注射卵清蛋白（OVA）建立AR小鼠模型。通过流式细胞术检测浸入鼻灌洗液中的免疫细胞。通过ELISA测定血清中的细胞因子浓度。打喷嚏和鼻擦事件的次数表明了AR小鼠的症状。在AR小鼠中，与对照小鼠相比，CCL2表达水平和CD45 ⁺ CD11b ⁺ Ly6C ^hi炎性单核细胞显着增加。CCL2 siRNA封装的纳米颗粒（NP _siCCL2）预防AR小鼠中CCL2表达和炎性单核细胞浸润。NP _siCCL2处理可显着减少AR小鼠的打喷嚏和鼻擦事件的数量。此外，NP _siCCL2处理可降低血清OVA特异性IgE，OVA特异性IgG1和组胺水平。在机械上，NP _siCCL2处理通过抑制Th2细胞因子（IL-4，IL-5和IL-13）的产生来减轻AR症状。纳米药物介导的炎性单核细胞浸润的预防可改善卵白蛋白诱发的小鼠过敏性鼻炎。