To investigate the yeast population dynamics during air exposure in total mixed ration (TMR) silage containing sweet potato residue. TMR were ensiled in laboratory silos (1 kg) with or without two lactic acid bacteria strains, Lactobacillus plantarum (LP), and Lactobacillus amylovorus (LA). Fermentation characteristics were measured and yeast population was investigated by ITS1 region gene sequencing using Illumina MiSeq platform. All treatments were well ensiled, and L. amylovorus improved aerobic stability. During aerobic exposure, Pichia kudriavzevii was detected with increased relative abundance in all treatments and more relative abundant in LP. Pichia fermentans was more relative abundant in control. Higher relative abundance of Pichia anomala was detected in deteriorating LP. The relative abundance of Pichia ohmeri increased during later aerobic exposure in the control and LA, with a significant increase in the count of yeast population. Despite Cryptococcus was detected more relative abundant during early stage of aerobic exposure, the yeast population was below the detection limit. Aerobic deterioration was characterized by an increase in operational taxonomic units of Pichia . High relative abundance of P. anomala and P. kudriavzevii made aerobic deterioration easier. Inhibition of P. fermentans might be an effective strategy for improving the aerobic stability to some instance.

中文翻译：

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甘薯渣全混合日粮空气暴露中酵母菌群动态变化。

研究含甘薯渣的全混合日粮 (TMR) 青贮饲料空气暴露过程中酵母菌种群动态变化。TMR 被青贮在实验室筒仓 (1 kg) 中，有或没有两种乳酸菌菌株，植物乳杆菌(LP) 和食淀粉乳杆菌(LA)。测量发酵特性并使用 Illumina MiSeq 平台通过 ITS1 区域基因测序研究酵母种群。所有处理都得到很好的青贮，食淀粉乳杆菌改善了有氧稳定性。在有氧暴露期间，Pichia kudriavzevii在所有处理中检测到相对丰度增加，在 LP 中检测到相对丰度更高。发酵毕赤酵母在控制上相对更丰富。在恶化的 LP 中检测到较高相对丰度的异常毕赤酵母。在对照和 LA 的后期有氧暴露过程中， Pichia ohmeri的相对丰度增加，酵母菌数量显着增加。尽管在有氧暴露的早期阶段检测到隐球菌相对较多，但酵母菌群低于检测限。有氧退化的特点是毕赤酵母的操作分类单位增加。P. anomala和P. kudriavzevii的高相对丰度使需氧降解更容易。抑制发酵假单胞菌在某些情况下可能是提高有氧稳定性的有效策略。