Human carcinoembryonic antigen‐related cell adhesion molecules (CEACAMs) are a family of receptors that mediate intercellular interactions. Pathogenic bacteria have ligands that bind CEACAMs on human cells. Neisseria gonorrhoeae (Gc) encodes numerous unique outer membrane opacity‐associated (Opa) proteins that are ligands for one or more CEACAMs. CEACAMs that are expressed on epithelial cells facilitate Gc colonization, while those expressed on neutrophils affect phagocytosis and consequent intracellular survival of Gc. Since Opa protein expression is phase‐variable, variations in receptor tropism affect how individual bacteria within a population interact with host cells. Here we report the development of a rapid, quantitative method for collecting and analyzing fluorescence intensity data from thousands of cells in a population using imaging flow cytometry to detect N‐CEACAM bound to the surface of Opa‐expressing Gc. We use this method to confirm previous findings regarding Opa‐CEACAM interactions and to examine the receptor–ligand interactions of Gc expressing other Opa proteins, as well as for other N‐CEACAM proteins. © 2020 International Society for Advancement of Cytometry

中文翻译：

---

CEACAM 与表达 Opa 的淋病奈瑟菌结合的成像流式细胞术分析。

人癌胚抗原相关细胞粘附分子 (CEACAM) 是介导细胞间相互作用的受体家族。致病细菌具有与人体细胞上的 CEACAM 结合的配体。淋球菌(Gc) 编码许多独特的外膜混浊相关 (Opa) 蛋白，这些蛋白是一种或多种 CEACAM 的配体。在上皮细胞上表达的 CEACAM 促进 Gc 定植，而在中性粒细胞上表达的 CEACAM 影响 Gc 的吞噬作用和随后的细胞内存活。由于 Opa 蛋白表达是相位可变的，受体向性的变化会影响种群中单个细菌与宿主细胞的相互作用。在这里，我们报告了一种快速、定量方法的开发，该方法使用成像流式细胞术检测与表达 Opa 的 Gc 表面结合的 N-CEACAM，用于收集和分析群体中数千个细胞的荧光强度数据。我们使用这种方法来证实先前关于 Opa-CEACAM 相互作用的发现，并检查表达其他 Opa 蛋白的 Gc 以及其他 N-CEACAM 蛋白的受体-配体相互作用。© 2020 国际细胞计量学促进会