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Ovarian development in swimming crabs: Comparative histochemistry and ultrastructure of Callinectes ornatus and Arenaeus cribrarius (Brachyura, Portunidae).
Tissue & Cell ( IF 2.6 ) Pub Date : 2020-06-02 , DOI: 10.1016/j.tice.2020.101395
M A Garcia Bento 1 , F A Nascimento 2 , F L Mantelatto 3 , F J Zara 1
Affiliation  

The ovarian development of Callinectes ornatus and Arenaeus cribrarius was described using histochemistry and ultrastructure. Both species shows the same ovarian stages, which are the juvenile (JUV), adult rudimentary (RUD), developing (DEV), intermediary (INT), mature (MAT), and spent (OV) stages. The JUV and RUD stages showed similar characteristics, and previtellogenesis is characterized by meiotic prophase chromosomes. In the primary vitellogenesis, the oocyte cytoplasm shows many small and large cytoplasmic glycoprotein vesicles. These vesicles correspond to the dilated cisternae of the rough endoplasmic reticulum (RER), which produces the immature (endogenous) yolk. Secondary vitellogenesis (exogenous phase) begins at the DEV stage with the fusion of pinocytic vesicles and vesicles with immature yolks to form mature yolk granules. At the INT stage, the formation of the chorion begins, and the mature yolks increase in size and number, while the RER diminishes. In the MAT stage, the oocytes are completely formed, and the cytoplasm is filled with mature yolk, lipid droplets, and glycogen. There are no significant variations between the gonadosomatic and hepatosomatic indices, which allows us to infer that the transfer of reserves from the hepatopancreas is nearly constant during ovarian development, since we observed primiparous and multiparous females in the same sampled population.



中文翻译:

游泳蟹的卵巢发育:Callinectes ornatus和Areneus cribrarius(Brachyura,Portunidae)的比较组织化学和超微结构。

Callinectes ornatusAreneus cribrarius的卵巢发育用组织化学和超微结构描述。两种物种都显示相同的卵巢阶段,分别是少年阶段(JUV),成年初级阶段(RUD),发育阶段(DEV),中间阶段(INT),成熟阶段(MAT)和花期(OV)。JUV和RUD阶段显示出相似的特征,并且玻璃体形成的特征在于减数分裂前期染色体。在初级卵黄发生中,卵母细胞的细胞质显示出许多大小的细胞质糖蛋白囊泡。这些囊泡对应于粗糙的内质网(RER)的膨胀水箱,产生未成熟的(内源)蛋黄。继发卵黄发生(外源期)始于DEV阶段,即胚泡囊泡和未成熟卵黄囊泡融合形成成熟卵黄颗粒。在INT阶段,绒毛膜的形成开始,成熟的蛋黄的大小和数量增加,而RER减小。在MAT阶段,卵母细胞完全形成,细胞质充满卵黄,脂质滴和糖原。性腺体和肝体指数之间没有显着差异,这使我们可以推断,在卵巢发育过程中,肝胰腺中储备的转移几乎是恒定的,因为我们在同一抽样人群中观察到初生和多胎雌性。

更新日期:2020-06-02
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