Abstract Considering the significant evidential values of fingerprints in underwater criminal investigations and the need to visualise them using a user- and environmentally-friendly reagent, development of a novel, rapid and relatively greener nanobio-based reagent (NBR) is deemed beneficial. Lipase from the commercial Candida rugosa immobilised onto acid-functionalised multi-walled carbon nanotubes (NBR) was used as the safer and cheap lipid-sensing reagent to visualise groomed whole/split fingerprints on non-porous objects immersed in stagnant tap water for up to 30 days under a laboratory-controlled setting. Attenuated Total Reflectance – Fourier Transform Spectrometry, Field Emission Scanning Electron Microscopy and bioinformatics (molecular docking and molecular dynamics simulations) were employed to characterise and confirm the attachment of NBR onto the lipid constituents of wet fingerprints. Chromatographic results further confirmed the presence of n-hexadecanoic and octadecanoic acids on fingerprints up to 30 days of immersion. Thus, NBR may potentially be useful as the future state-of-the-art fingerprint visualisation technology.

中文翻译：

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一种新型脂肪酶纳米生物试剂的表征和计算分析，用于可视化水浸载玻片上的潜在指纹

摘要 考虑到指纹在水下刑事调查中的重要证据价值以及使用用户和环境友好的试剂将它们可视化的需要，开发一种新型、快速且相对绿色的纳米生物基试剂 (NBR) 被认为是有益的。来自固定在酸官能化多壁碳纳米管 (NBR) 上的商业化假丝酵母的脂肪酶被用作更安全、更便宜的脂质传感试剂，以可视化浸没在停滞自来水中的无孔物体上修饰的完整/分裂指纹在实验室控制的环境下 30 天。衰减全反射 - 傅立叶变换光谱法，场发射扫描电子显微镜和生物信息学（分子对接和分子动力学模拟）被用来表征和确认 NBR 附着在湿指纹的脂质成分上。色谱结果进一步证实，浸泡 30 天后，指纹上存在正十六烷酸和十八烷酸。因此，NBR 可能作为未来最先进的指纹可视化技术有用。