Three-dimensional (3D) printing technology has attracted considerable focus for preparing porous bone repair scaffolds to promote bone regeneration. Inspired by organic-inorganic components and the porous structure of natural bone, novel porous degradable scaffolds have been printed using hydroxyapatite (HA), carboxymethyl chitosan (CMCS), and polydopamine (PDA). The well-designed HA/CMCS/PDA scaffolds exhibited a porous structure with 60.5 ± 4.6% porosity and 415 ± 87 μm in mode pore diameter. The weight loss percentage (WL%) of the HA/CMCS/PDA scaffolds reached about 17% during a 10-week degradation in vitro. The degradation process between the CMCS and HA induced the release of calcium ions. Using commercial product as the contrast material, the osteogenic properties of the scaffolds were assessed in vivo. The implantation and degradation of HA/CMCS/PDA scaffolds had no adverse effects on the kidney and liver of rabbits with no inflammatory response in the implantation sites. The micro-CT and histology data suggested that the HA/CMCS/PDA scaffolds could effectively stimulate new bone formation within the femoral lacuna defect region of rabbits versus blank control at 12 weeks after implantation. Surface cortical bone was generated in the defect area in the HA/CMCS/PDA group; the defect in the blank group remained obvious. HA/CMCS/PDA scaffolds had excellent biodegradability matching the formation of new bone during implantation. In conclusion, 3D-printed HA/CMCS/PDA scaffolds have remarkable potential as a new material for repairing bone defects.

三维（3D）打印技术已经吸引了相当多的注意力，用于制备多孔骨修复支架以促进骨再生。受有机无机成分和天然骨的多孔结构的启发，新型多孔可降解支架使用羟基磷灰石（HA），羧甲基壳聚糖（CMCS）和聚多巴胺（PDA）进行印刷。精心设计的HA / CMCS / PDA支架显示出多孔结构，孔隙率为60.5±4.6％，众数孔径为415±87μm。在10周的体外降解过程中，HA / CMCS / PDA支架的失重百分比（WL％）达到了约17％。CMCS和HA之间的降解过程诱导了钙离子的释放。使用商品作为对比材料，评估支架的成骨特性体内。HA / CMCS / PDA支架的植入和降解对兔子的肾脏和肝脏没有不良影响，并且在植入部位没有炎症反应。显微CT和组织学数据表明，与空白对照组相比，HA / CMCS / PDA支架可以在植入后12周有效刺激兔子股骨缺损区域内的新骨形成。HA / CMCS / PDA组的缺损区域产生了表面皮质骨。空白组的缺陷仍然很明显。HA / CMCS / PDA支架具有出色的生物降解能力，与植入过程中新骨的形成相匹配。总之，3D打印的HA / CMCS / PDA支架作为修复骨缺损的新材料具有巨大的潜力。