Characterisation of the entire genome of Fowl aviadenoviruses (FAdV) requires isolation and propagation of the virus in chicken embryo liver or kidney cells, a process which is not only time consuming but may occasionally fail to result in viral growth. Furthermore, in a mixed infection, isolation in cell culture may result in the loss of viral strains. In this study, we optimised a FAdV DNA extraction technique directly from affected liver tissues using kaolin hydrated aluminium silicate treatment. The whole genome of FAdV was sequenced directly from extracted DNA without any targetted PCR based enrichment. The extraction method was also tested on avian liver tissues affected with the RNA virus Avian hepatitis E virus and demonstrated to yield sequencing grade RNA. Therefore, the method described here is a simple technique which is potentially useful for the extraction of sequencing grade DNA/RNA from tissues with high fat content.

禽禽腺病毒（FAdV）整个基因组的表征需要在鸡胚肝或肾细胞中分离和传播该病毒，这一过程不仅费时，而且有时无法导致病毒生长。此外，在混合感染中，细胞培养物中的分离可能导致病毒株的损失。在这项研究中，我们使用高岭土水合硅酸铝处理直接从受影响的肝脏组织中优化了FAdV DNA提取技术。直接从提取的DNA测序FAdV的整个基因组，无需任何基于PCR的靶向富集。还对受RNA病毒禽E型肝炎病毒影响的禽肝组织测试了提取方法，并证明其可产生测序级RNA。因此，