Abstract—

The search for effective and nontoxic sterilization drugs for plants against common phytopathogenic microorganisms is a major challenge to improve the biotechnology of plant clonal micropropagation. An analysis of 92 studies that describe the potential use of ZnO and TiO₂ nanoparticles as antimicrobial agents in biotechnology showed that their biological effects depend on several factors: photocatalytic activity, particle size, concentration, morphology, and surface modification. The mechanisms of toxicity, among which the primary one is generation of reactive oxygen species leading to oxidative stress, are also due to these factors. The data describing the direct influence of ZnO and TiO₂ nanoparticles on plants, however, are contradictory, which is probably because of the various particle shapes and sizes, their concentrations, and the species characteristics of the plants studied. These studies have confirmed that photocatalytically active ZnO and TiO₂ nanoparticles may be used as bactericidal and fungicidal drugs for sterilization of explants during clonal micropropagation of plants, while taking into account the possible phytotoxicity of these particles.

中文翻译：

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光催化活性氧化锌和二氧化钛纳米粒子在植物的克隆微繁殖中的应用前景

摘要—

寻找有效的，无毒的针对常见植物病原微生物的植物杀菌药物，是提高植物克隆微繁殖生物技术的主要挑战。对92项描述了ZnO和TiO ₂纳米颗粒作为抗微生物剂在生物技术中的潜在用途的研究的分析表明，它们的生物学效应取决于几个因素：光催化活性，粒径，浓度，形态和表面改性。毒性机理也归因于这些因素，其中主要的机理是导致氧化应激的活性氧的产生。描述ZnO和TiO ₂直接影响的数据然而，植物上的纳米颗粒是矛盾的，这可能是由于所研究植物的各种颗粒形状和大小，其浓度以及物种特征所致。这些研究已经证实，光催化活性的ZnO和TiO ₂纳米颗粒可以用作植物克隆无性繁殖过程中外植体杀菌的杀菌和杀真菌药物，同时考虑到这些颗粒的可能的植物毒性。