Nanowire SOI biosensors are used to detect the Ebola virus VP40 protein by identifying its immune complexes with specific monoclonal antibodies (MCAs). It is shown that the reaction of specific interaction between VP40 and MCAs causes a more pronounced biosensor response than direct adsorption of VP40 or antibodies to their surface. The formation of antigen — antibody complexes leads to the accumulation of a negative charge on the biosensor surface. The protein and protein — antibody complexes are detected in real time (∼200–300 s per test sample).

中文翻译：

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使用纳米线 SOI 生物传感器检测埃博拉病毒 VP40 蛋白

纳米线 SOI 生物传感器用于通过识别具有特定单克隆抗体 (MCA) 的免疫复合物来检测埃博拉病毒 VP40 蛋白。结果表明，与 VP40 或抗体直接吸附到其表面相比，VP40 和 MCA 之间特定相互作用的反应会引起更明显的生物传感器响应。抗原-抗体复合物的形成导致生物传感器表面积累负电荷。实时检测蛋白质和蛋白质 - 抗体复合物（每个测试样品约 200-300 秒）。