Abstract

Long-distance interaction plays an important role in the regulation of eukaryotic genes. Chromatin structure is involved in the process, but the role of histone modifications has not been studied. In the present work, the role of acetylation Н4K16 (Н4K16-Ac) to enhancer-promoter communication (EPC) was analyzed. This modification is associated with euchromatin and is involved in the decompaction of chromatin fibers. We have shown that the effect of H4K16-Ac on EPC in vitro depends on the level of chromatin assembly. EPC in chromatin, which lacks nucleosomes at random positions on DNA, is inhibited in the presence of H4K16-Aс. At the same time, EPC in chromatin, in which nucleosomes occupy all available positions on DNA, is somewhat stimulated in the presence of H4K16-Aс.

中文翻译：

---

组蛋白H4乙酰化对染色质通讯的影响

摘要

远距离相互作用在真核基因的调控中起着重要作用。染色质结构参与该过程，但尚未研究组蛋白修饰的作用。在本工作中，分析了乙酰化Н4K16（Н4K16-Ac）在增强子-促进子通讯（EPC）中的作用。这种修饰与常染色质相关，并参与染色质纤维的分解。我们已经表明，H4K16-Ac在体外EPC上的作用取决于染色质装配的水平。在H4K16-Aс的存在下，染色质中的EPC（在DNA的随机位置缺少核小体）受到抑制。同时，在H4K16-Aс的存在下，染色质中的EPC（其中核小体占据了DNA上所有可用的位置）受到了一定程度的刺激。