Abstract

The universal response of Escherichia coli to stress is enhancing the synthesis of specific histone-like Dps proteins that bind bacterial DNA. As a result, two-dimensional and three-dimensional crystalline arrays can be observed in the cytoplasm of starving bacteria. Conditions for obtaining in vitro co-crystals of DNA-Dps were selected, and their elemental composition was studied using analytical electron microscopy. It was found that Dps in the co-crystal retains its ferritin-like activity; that is, it can stimulate the oxidation of Fe²⁺ ions to Fe³⁺ and facilitate the accumulation of iron in the form of Fe₂O₃ in the inner cavity of the oligomer.

中文翻译：

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DNA-蛋白质晶体的元素组成体外分析

摘要

大肠杆菌对压力的普遍反应正在增强结合细菌DNA的特定组蛋白样Dps蛋白的合成。结果，可以在饥饿的细菌的细胞质中观察到二维和三维晶体阵列。选择获得DNA-Dps体外共晶体的条件，并使用分析电子显微镜研究其元素组成。发现共晶体中的Dps保留了其铁蛋白样活性。也就是说，它可以刺激Fe ²⁺离子氧化为Fe ³⁺并促进铁以Fe ₂ O ₃的形式在低聚物的内腔中积累。