Abstract—

A search for geroprotectors that would affect the telomere length and/or telomerase activity and not exhibit tumorigenicity is under progress. The medical use of various extracts of plants from the Astragalus genus of the Fabaceae family was first described in 200 CE. However, scientific studies of the components of these extracts (cycloastragenol, astragaloside IV) have begun only recently. The goal of this work was to explore the effect of a composition consisting of astragaloside IV, cycloastragenol, and the Timovial–Epivial dipeptide and its components on telomere length and telomerase activity in human umbilical cord mesenchymal stromal cells and aging fibroblasts. Telomerase activity was determined by the telomeric repeat amplification protocol (TRAP). Telomere length was determined by measuring the intensity of fluorescence in situ hybridization signals using flow cytometry. None of the composition components caused a significant change in the determined parameters on its own. Only cycloastragenol had a negligible effect on the telomere length in fibroblasts. The combination of the key compound, astragaloside IV, aglycone of astragalosides—cycloastragenol (CAG), and biopeptide complex produced more significant changes in the telomere length and telomerase activity than did each component alone. The complex was shown to have a significant effect on telomere length and telomerase activity. Further studies are needed to determine the mechanism of the effect of the components combination on telomere length and telomerase activity.

中文翻译：

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代孕激素保护剂黄芪甲苷IV，环雌三醇和上皮-表位肽复合物对人间充质基质细胞和衰老成纤维细胞端粒长度和端粒酶活性的影响

摘要-

正在寻找能影响端粒长度和/或端粒酶活性并且不表现出致瘤性的神经保护剂。早在200 CE中就描述了来自豆科的黄芪属的各种植物提取物的医学用途。但是，这些提取物（环黄芪醇，黄芪甲苷IV）的成分的科学研究直到最近才开始。这项工作的目的是探讨由黄芪甲苷IV，环黄芪醇和Timovial-Epivial二肽及其成分组成的组合物对人脐带间充质基质细胞和衰老的成纤维细胞端粒长度和端粒酶活性的影响。端粒酶活性通过端粒重复扩增方案（TRAP）确定。通过使用流式细胞仪测量荧光原位杂交信号的强度来确定端粒的长度。没有组成成分会单独导致确定的参数发生重大变化。只有环黄芪醇对成纤维细胞端粒长度的影响可忽略不计。与单独使用每种成分相比，关键化合物，黄芪甲苷IV，黄芪总苷的糖苷-环黄芪醇（CAG）和生物肽复合物的组合在端粒长度和端粒酶活性方面产生了更显着的变化。已显示该复合物对端粒长度和端粒酶活性具有显着影响。需要进一步的研究以确定组分组合对端粒长度和端粒酶活性的作用机理。没有组成成分会单独导致确定的参数发生重大变化。只有环黄芪醇对成纤维细胞端粒长度的影响可忽略不计。与单独使用每种成分相比，关键化合物，黄芪甲苷IV，黄芪总苷苷-环黄芪醇（CAG）和生物肽复合物的组合在端粒长度和端粒酶活性方面产生了更显着的变化。已显示该复合物对端粒长度和端粒酶活性具有显着影响。需要进一步的研究以确定组分组合对端粒长度和端粒酶活性的作用机理。没有组成成分会单独导致确定的参数发生重大变化。只有环黄芪醇对成纤维细胞端粒长度的影响可忽略不计。与单独使用每种成分相比，关键化合物，黄芪甲苷IV，黄芪总苷的糖苷-环黄芪醇（CAG）和生物肽复合物的组合在端粒长度和端粒酶活性方面产生了更显着的变化。已显示该复合物对端粒长度和端粒酶活性具有显着影响。需要进一步的研究以确定组分组合对端粒长度和端粒酶活性的作用机理。与单独使用每种成分相比，黄芪甲苷IV，黄芪甲苷-环黄芪醇（CAG）的糖苷配基和生物肽复合物在端粒长度和端粒酶活性方面产生了更显着的变化。已显示该复合物对端粒长度和端粒酶活性具有显着影响。需要进一步的研究以确定组分组合对端粒长度和端粒酶活性的作用机理。与单独使用每种成分相比，黄芪甲苷IV，黄芪甲苷-环黄芪醇（CAG）的糖苷配基和生物肽复合物在端粒长度和端粒酶活性方面产生了更显着的变化。已显示该复合物对端粒长度和端粒酶活性具有显着影响。需要进一步的研究以确定组分组合对端粒长度和端粒酶活性的作用机理。