Abstract

Endometriosis is a widespread disorder in which a tissue morphologically and physiologically similar to endometrium is observed outside the uterine cavity. Earlier, we identified a number of genes that are overexpressed in ectopic endometrium compared to eutopic endometrium. C7, FABP4, ADH1B, and PLA2G2A were the most expressed genes. The difference in their expression level was 200–300 times higher. In this work, we verified the results using real-time PCR and immunohistochemistry and examined the expression of these genes in the peritoneum of patients with endometriosis and healthy women. We have shown that a high level of expression of these genes is due to endometriotic lesions and peritoneal cells rather than eutopic endometrium. The proteins encoded by these genes (FABP4, PLA2G2A, and ADH1B) are known to be adipogenesis markers. Protein C7 is also involved in the differentiation of adipose stem cells. The equally high level of expression of these genes in endometriosis nodules and underlying peritoneum may point to a common origin of these tissues, which supports the metaplastic theory of the origin of endometriosis. In addition, our data can be interpreted as an indication that adipogenic stem cells may play a role in the pathogenesis of endometriosis. Also, our data can be interpreted as an indication of a possible involvement of adipogenic stem cells in the pathogenesis of endometriosis.

中文翻译：

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子宫内膜异位病变中脂肪形成蛋白标志物的表达

摘要

子宫内膜异位症是一种广泛的疾病，其中在子宫腔外观察到与子宫内膜形态和生理相似的组织。早些时候，我们确定了与异位子宫内膜相比在异位子宫内膜中过表达的许多基因。C7，FABP4，ADH1B和PLA2G2A是表达最多的基因。它们的表达水平差异高200-300倍。在这项工作中，我们使用实时PCR和免疫组化方法验证了结果，并检查了这些基因在子宫内膜异位症患者和健康女性腹膜中的表达。我们已经证明这些基因的高水平表达是由于子宫内膜异位病变和腹膜细胞而不是异位子宫内膜引起的。这些基因（FABP4，PLA2G2A和ADH1B）编码的蛋白质是成脂标记。蛋白C7也参与脂肪干细胞的分化。这些基因在子宫内膜异位症结节和潜在的腹膜中的表达水平较高，可能表明这些组织的共同起源，这支持子宫内膜异位症起源的化生理论。此外，我们的数据可以解释为成脂干细胞可能在子宫内膜异位症发病机制中起作用的迹象。同样，我们的数据可以解释为成脂干细胞可能参与子宫内膜异位症发病机制的迹象。