Abstract

The aim of this study was to determine the structural–morphological differences between rapid and classic netosis of human neutrophils. A method for studying the netosis process using lattices for transmission electron microscopy has been developed. Dynamics of blood neutrophil netosis and differences in the cell death processes induced by ionomycin and opsonized bacteria Staphylococcus aureus were studied with flow cytometry. Morphological features of rapid and classic netosis were examined with methods of scanning electron, fluorescence, and light microscopy. The structural and morphological similarities and differences between the two variants of netosis induced by opsonized S. aureus were described. It was revealed that classical netosis can induce the formation of extracellular traps in neighboring neutrophils. It was shown that the morphological features of extracellular traps can be used to differentially diagnose the development of rapid and classical netosis not only with high-resolution microscopy, but also with traditional light microscopy.

中文翻译：

---

快速经典性netosis的形态特征

摘要

这项研究的目的是确定人类嗜中性粒细胞快速和经典netosis之间的结构-形态差异。已经开发出一种研究用于使用透射电子显微镜的晶格进行的netosis过程的方法。用流式细胞仪研究了离子霉素和调理细菌金黄色葡萄球菌引起的血液中性粒细胞netosis动态变化和细胞死亡过程的差异。快速和经典netosis的形态学特征用扫描电子，荧光和光学显微镜的方法进行了检查。调理过的金黄色葡萄球菌诱发的两个Netosis变种之间的结构和形态相似性和差异被描述。揭示了经典的netosis可以在邻近的中性粒细胞中诱导细胞外陷阱的形成。结果表明，细胞外捕获物的形态学特征不仅可以通过高分辨率显微镜，而且可以通过传统的光学显微镜，用于鉴别诊断快速和经典的netosis。