Abstract —Atomic force microscopy was used to create a system for measuring intercellular adhesion. Scanning electron microscopy was applied to confirm that poly-L-lysine is the best substance for fixation of neutrophils and lymphocytes on the probe. It was shown that when one cell is fixed on the probe and the other one is fixed on the substrate surface it is possible to carry out relevant measurements of intercellular adhesion forces by FS spectroscopy. The intercellular adhesion forces of neutrophils and lymphocytes were measured. The adhesion forces of neutrophils and lymphocytes were 38.5 ± 13.7 pN and 35.6 ± 8.3 pN, respectively. Thus, adhesion of neutrophils is statistically significantly higher than adhesion of lymphocytes.

中文翻译：

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用原子力显微镜研究中性粒细胞和淋巴细胞的粘附细胞间接触

摘要 —原子力显微镜用于创建测量细胞间粘附的系统。应用扫描电子显微镜证实聚-L-赖氨酸是将嗜中性粒细胞和淋巴细胞固定在探针上的最佳物质。结果表明，当一个细胞固定在探针上而另一个细胞固定在基板表面时，可以通过FS光谱进行细胞间粘附力的相关测量。测量中性粒细胞和淋巴细胞的细胞间粘附力。中性粒细胞和淋巴细胞的粘附力分别为 38.5 ± 13.7 pN 和 35.6 ± 8.3 pN。因此，嗜中性粒细胞的粘附在统计学上显着高于淋巴细胞的粘附。