Abstract

The dynamics of the development of immobilized cells in a gel under conditions of limited mass transfer of nutrients across the interface was considered. The goal of this study was to find methods for effective cultivation of living microorganisms in the gel due to the correct choice of the structure of internal channels, when nutrients are delivered to the gel by the convective-diffusion mechanism. The variation of the relative intensity of transmitted light along the height of the agarose gel sample with living yeast cells during nutrient solution diffusion from the surface with time was determined by spectroscopy. The depth of the gel layer at which the vital activity of microorganisms is provided was evaluated. The possibility of forming time-stable linear and branching channels in the gel was established experimentally. The possibility of their stable use for convective supply of nutrient in the volume was verified. The tendencies of diffusion of nutrients from the channels in the gel with immobilized cells were determined. The 3D structure of channels that provides an increase in the efficiency of cell nutrition in the gel volume was discussed.

中文翻译：

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营养物质在具有不同空间结构通道的凝胶中的非稳态传质

摘要

考虑了在界面上营养物质的质量转移受限的条件下，凝胶中固定化细胞发育的动力学。这项研究的目的是找到通过对流扩散机制将营养物质输送到凝胶中时，由于内部通道结构的正确选择，从而有效地在凝胶中培养活微生物的方法。在营养液从表面扩散的过程中，通过活的酵母细胞，透射光的相对强度沿琼脂糖凝胶样品的高度随活酵母细胞的变化随时间变化。评估提供微生物生命活动的凝胶层的深度。通过实验确定了在凝胶中形成时间稳定的线性和分支通道的可能性。验证了它们稳定用于大量对流营养供应的可能性。确定了养分从固定化细胞的凝胶中的通道扩散出来的趋势。讨论了在凝胶体积中增加细胞营养效率的通道的3D结构。