Abstract

Plant cysteine-rich peptides (CRP) play a key role in protection of plants from pathogens and abiotic stress, in symbiosis with bacteria, and in plant development and reproduction. The composition and role of CRPs in tomato Solanum lycopersicum, one of the most important vegetable, has not been studied so far. In this paper, using two approaches, hidden Markov models and regular expressions, in silico identification of tomato CRP families was performed. A bioinformatic analysis of NCBI databases revealed 191 CRP precursors. The peptides found belong to the families of antimicrobial and signaling peptides. Among the antimicrobial peptides, defensins and defensin-like peptides, nonspecific lipid-transfer proteins, thionins, snakins, and hevein- and knottin-like peptides were found. Among signaling peptides, the peptides belonging to the RALF, Ole e 1, Ole e 6, and MEG families were discovered. In addition, peptides with new cysteine motifs were identified. All discovered CRPs are synthesized as pre- or preproproteins. The predicted mature peptides were characterized by cysteine motifs, antimicrobial activity, and domain structure. Thus, for the first time, using bioinformatic approaches, systemic data on the arsenal of CRPs in the tomato genome were obtained, which creates a basis for further functional studies of these peptides and their subsequent use in agriculture to develop new strategies for increasing tomato pathogen resistance, as well as in medicine to create next-generation drugs.

中文翻译：

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在计算机上鉴定茄科茄中富含半胱氨酸肽的基因家族。

摘要

富含植物半胱氨酸的肽（CRP）在保护植物免受病原体和非生物胁迫，与细菌共生以及植物发育和繁殖中发挥关键作用。CRP在番茄茄中的组成和作用作为最重要的蔬菜之一，到目前为止尚未进行过研究。本文采用隐马尔可夫模型和正则表达式两种方法对番茄CRP家族进行了计算机鉴定。NCBI数据库的生物信息学分析显示191个CRP前体。发现的肽属于抗菌肽和信号肽家族。在抗微生物肽，防御素和防御素样肽中，发现了非特异性脂质转移蛋白，硫磷素，突触核蛋白以及类肝素和结蛋白样肽。在信号肽中，发现了属于RALF，Ole e1，Ole e 6和MEG家族的肽。另外，鉴定了具有新的半胱氨酸基序的肽。所有发现的CRP均合成为前蛋白或前蛋白。预测的成熟肽的特征是半胱氨酸基序，抗菌活性和域结构。因此，首次使用生物信息学方法获得了番茄基因组中CRP的砷库的系统数据，这为进一步研究这些肽及其在农业上的应用奠定了基础，以开发增加番茄病原体的新策略。抵抗力，以及在医学上创造下一代药物。