Abstract

The ITS1–5.8S rDNA–ITS2 sequence of the 35S rRNA genes of 16 species of feather grasses and 2 species of false needlegrasses of the flora of the Altai Republic and Altai krai (Stipabaicalensis, S. borysthenica, S. capillata, S. consanguinea, S. dasyphylla, S. desertorum, S. glareosa, S. grandis, S. korshinskyi, S. krylovii, S. lessingiana, S. orientalis,S. pennata, S. praecapillata, S. pulcherrima, S. zalesskii, Ptilagrostisjunatovii, and P. mongholica), as well as four feather grass species from other regions of Russia (S. pontica, S. rubens, S. tirsa, and S. ucrainica), was sequenced. The trnL-trnF chloroplast sequences of S. capillata, S. borysthenica, S. glareosa, S. krylovii, S. lessingiana, S. orientalis, S. pulcherrima, and S. zalesskii were also determined. The trnL-trnF region, as well as the 5.8S rDNA, is highly conserved in feather grasses and cannot be used to differentiate sections and species within the genus. The ITS1 and ITS2 sequences, on the contrary, have some parsimony-informative substitutions and indels, which makes it possible to identify groups of ribotypes corresponding to sections and individual species within some sections. The clades corresponding to the Stipa and Leiostipa sections were separated; the species of the Barbatae and Subbarbatae sections composed the same clade, while the section Hemibarbatae turned out to be polyphyletic. Only in the section Stipa were no species-specific nucleotide substitutions and indels revealed. The isolation of the species S. desertorum, the taxonomic rank of which was previously unclear, was shown. The origin of polymorphic sites in the ITS sequences of feather grasses is discussed as possible evidence of distant hybridization.

中文翻译：

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ITS1-5.8S rDNA-ITS2和trn L-trn F序列作为研究阿尔泰羽毛草物种多样性的标志

摘要

阿尔泰共和国和阿尔泰草原（Stipa baicalensis，S. borysthenica，S. capillata，S.）的16种针茅和2种假针叶草的35S rRNA基因的ITS1-5.8S rDNA-ITS2序列。 consanguinea，S.冰藜，S.沙生，S. glareosa，针茅，S. korshinskyi，克氏针茅，S. lessingiana，S.侧柏，S.羽状，S. praecapillata，S.一品红，S. zalesskii，细柄junatovii和P. mongholica），以及来自俄罗斯其他地区的四位羽毛草种（S. pontica，S.鲁本斯，S. tirsa和S. ucrainica），进行测序。在TRN L- TRN ˚F叶绿体序列S. capillata，S. borysthenica，S. glareosa，克氏针茅，S. lessingiana，S.侧柏，S.一品红，及S. zalesskii也进行了测定。该TRN L- TRNF区和5.8S rDNA在羽毛草中高度保守，不能用于区分属内的部分和物种。相反，ITS1和ITS2序列具有一些简约信息替换和插入缺失，这使得可以识别与部分相对应的核糖型组以及某些部分中的单个物种。对应于Stipa和Leiostipa部分的分支被分离；所述的种类半枝莲和Subbarbatae部分由相同的进化枝，而部分Hemibarbatae原来是多系。仅在Stipa部分没有发现物种特异性的核苷酸取代和插入缺失。显示了对沙门氏菌（S. desertorum）物种的分离，该物种的分类学等级以前不清楚。讨论了羽毛草ITS序列中多态性位点的起源，作为远距离杂交的可能证据。