Alzheimer’s disease (AD) is the most common neurodegenerative disease, and there has been no disease-modifying treatment for AD. Recent studies suggest that trehalose may have beneficial effect on neurodegenerative diseases through regulating autophagy and facilitating aggregated protein clearance. However, the effects of trehalose on AD-related neuropathologies are still unknown. Western blot was performed to examine the effects of trehalose on APP processing in vitro and in vivo. ELISA and immunohistochemical staining were conducted to measure Aβ production in vitro and neuritic plaque formation in APP23 transgenic mice, respectively. Trehalose treatment significantly decreased Aβ generation in HAW and 20E2 cells. Furthermore, trehalose treatment increased the levels of APP and its CTFs, and significantly reduced Aβ generation and neuritic plaque formation in APP23 mice. Our study showed that trehalose affected the APP processing both in vitro and in vivo and suggests that trehalose treatment may offer as a therapeutic strategy to ameliorate AD pathology by inhibiting Aβ generation and neuritic plaque formation.

阿尔茨海默氏病（AD）是最常见的神经退行性疾病，目前尚无可改善AD的疾病。最近的研究表明，海藻糖可能通过调节自噬和促进聚集蛋白清除而对神经退行性疾病产生有益作用。然而，海藻糖对AD相关的神经病理学的影响仍然未知。进行了蛋白质印迹以检查海藻糖在体外和体内对APP加工的影响。进行了ELISA和免疫组化染色以分别测量APP23转基因小鼠的Aβ产生量和神经噬菌斑的形成。海藻糖处理显着降低了HAW和20E2细胞中Aβ的生成。此外，海藻糖治疗可提高APP及其CTF的水平，并显着降低APP23小鼠的Aβ生成和神经斑块形成。我们的研究表明，海藻糖在体外和体内均会影响APP的加工过程，并表明海藻糖治疗可能通过抑制Aβ的生成和神经斑的形成来改善AD的病理状态。