As the largest family among the plant resistance (R) proteins, the nucleotide-binding site-leucine-rich repeat (NBS-LRR) proteins play significant roles in the defense of pathogens. The completion and improvement of banana reference sequence make a systematic insight into the banana NBS-LRR protein family possible. In this study, a total of 98 NBS-LRR proteins were identified from the banana genome and clustered into eight classes in the phylogenetic tree. NBS-LRR genes were unevenly distributed on all 11 chromosomes of banana. Typical NB-ARC (nucleotide binding-APAF-1, disease resistance proteins, CED-4) and LRR motifs were found in all NBS-LRR proteins and a small number of introns existed in most NBS-LRR genes. Transcriptional profiles of NBS-LRRs were investigated in Fusarium oxysporum f. sp. cubense (Foc) susceptible and resistant banana cultivars BX and HDJ challenged with Foc race 1 (Foc 1) and tropical race 4 (Foc TR4). Among 31 representative NBS-LRRs detected in this study, 12 members showed stronger transcriptional stimulation in HDJ than in BX, while in contrast, eight members exhibited higher expression in BX than in HDJ. This study advances our understanding of roles of banana NBS-LRRs in the defense of Fusarium wilt and will contribute to the genetic improvement of banana resistance.

作为植物抗性（R）蛋白中最大的家族，核苷酸结合位点-富含亮氨酸的重复序列（NBS-LRR）蛋白在病原体防御中起着重要作用。香蕉参考序列的完成和改进使对香蕉NBS-LRR蛋白家族的系统了解成为可能。在这项研究中，从香蕉基因组中鉴定出总共98种NBS-LRR蛋白，并在系统发育树中分为8类。NBS-LRR基因在香蕉的所有11条染色体上分布不均。在所有NBS-LRR蛋白中都发现了典型的NB-ARC（核苷酸结合-APAF-1，抗病蛋白，CED-4）和LRR基序，大多数NBS-LRR基因中存在少量内含子。NBS-LRR的转录谱在尖孢镰刀菌f中进行了调查。sp。香蕉枯萎病（FOC）敏感和抗性品种香蕉BX和HDJ挑战与FOC种族1（FOC 1）和热带种族4（FOC TR4）。在这项研究中检测到的31个代表性NBS-LRR中，有12个成员在HDJ中表现出比BX更强的转录刺激，而相反，有8个成员在BX中表现出比HDJ中更高的表达。这项研究提高了我们对香蕉NBS-LRR在防御枯萎病中的作用的理解，并将有助于香蕉抗性的遗传改良。