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FTIR analysis of pyrogallol and phytotoxicity-reductive effect against mercury chloride.
Environmental Geochemistry and Health ( IF 3.2 ) Pub Date : 2020-06-02 , DOI: 10.1007/s10653-020-00607-1
Janaina E Rocha 1 , Tássia T A M Guedes 1 , Camila F Bezerra 1 , Maria do S Costa 1 , Fabia F Campina 1 , Thiago S de Freitas 1 , Amanda K Sousa 1 , Celestina E Sobral Souza 1 , Maria K N Silva 1 , Yedda M Lobo 1 , Francisco N Pereira-Junior 2 , João H da Silva 2 , Irwin R A Menezes 3 , Raimundo N P Teixeira 4 , Aracélio V Colares 5 , Henrique D M Coutinho 1
Affiliation  

Human activities, especially in industry, have contributed to soil contamination with heavy or toxic metals. The objective of this study was to determine the chelating effect and antioxidant activity of pyrogallol, as well as to evaluate its cytoprotective activity in prokaryotic and eukaryotic models, animal and plant, respectively, against toxic mercury chloride action. Antioxidant activity was determined by DPPH where pyrogallol showed considerable action, chelating even iron ions. For the microbiologic activity assays, microdilution was performed to obtain the minimal inhibitory concentration, minimum bactericidal and minimum fungicide concentration, from which the sub-inhibitory concentrations were determined. The product did not conferred cytoprotection to the tested bacteria and fungi. To evaluate plant cytoprotection, Lactuta sativa seeds were used together with the product at a sub-allelopathic concentration with different HgCl2 concentrations. In this case, the tannin conferred cytoprotection to the plant model, allowing the best growth and development of caulicles and radicles, thus preserving tissues necessary for plant survival. From the results, it is observable that pyrogallol possesses cytoprotective action in the eukaryotic plant model, this action being useful as an alternative which favors the growth of plants in contaminated areas, as the recovering of crop fields or reforestation projects.



中文翻译:


连苯三酚的 FTIR 分析及其对氯化汞的药害降低作用。



人类活动,特别是工业活动,导致土壤受到重金属或有毒金属的污染。本研究的目的是确定连苯三酚的螯合作用和抗氧化活性,并评估其分别在原核和真核模型、动物和植物中针对有毒氯化汞作用的细胞保护活性。抗氧化活性通过 DPPH 测定,其中连苯三酚表现出相当大的作用,甚至可以螯合铁离子。对于微生物活性测定,进行微量稀释以获得最小抑菌浓度、最小杀菌剂和最小杀真菌剂浓度,由此确定亚抑菌浓度。该产品没有对测试的细菌和真菌产生细胞保护作用。为了评价植物细胞保护作用,将Lactuta sativa种子与具有不同HgCl 2浓度的亚化感浓度的产品一起使用。在这种情况下,单宁赋予植物模型细胞保护作用,使花茎和胚根能够最佳生长和发育,从而保存植物生存所需的组织。从结果中可以看出,连苯三酚在真核植物模型中具有细胞保护作用,这种作用可作为有利于污染地区植物生长的替代方案,如农田恢复或重新造林项目。

更新日期:2020-06-02
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