The Xanthomonadaceae family comprises the genera Xanthomonas and Xylella , which include plant pathogenic species that affect economically important crops. The family also includes the plant growth-promoting bacteria Pseudomonas geniculata and Stenotrophomonas rhizophila , and some other species with biotechnological, medical, and environmental relevance. Previous work identified molecular signatures that helped to understand the evolutionary placement of this family within gamma-proteobacteria. In the present study, we investigated whether insertions identified in highly conserved proteins may also be used as molecular markers for taxonomic classification and identification of members within the Xanthomonadaceae family. Four housekeeping proteins (DNA repair and replication-related and protein translation enzymes) were selected. The insertions allowed discriminating phytopathogenic and plant growth-promoting groups within this family, and also amino acid sequences of these insertions allowed distinguishing different genera and, eventually, species as well as pathovars. Moreover, insertions in the proteins MutS and DNA polymerase III (subunit alpha) are conserved in Xylella fastidiosa , but signatures in DNA ligase NAD-dependent and Valyl tRNA synthetase distinguish particular subspecies within the genus. The genus Stenotrophomonas and Pseudomonas geniculata could be distinguishable based on the insertions in MutS, DNA polymerase III (subunit alpha), and Valyl tRNA synthetase, although insertion in DNA ligase NAD-dependent discriminates these bacteria at the species level. All these insertions differentiate species and pathovars within Xanthomonas . Thus, the insertions presented support evolutionary demarcation within Xanthomonadaceae and provide tools for the fast identification in the field of these bacteria with agricultural, environmental, and economic relevance.

中文翻译：

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鉴定黄单胞菌科不同属的蛋白质特征

黄单胞菌科包括黄单胞菌属和木霉属，其中包括影响经济重要作物的植物病原物种。该家族还包括促进植物生长的细菌 Pseudomonas geniculata 和 Stenotrophomonas rhizophila，以及其他一些与生物技术、医学和环境相关的物种。以前的工作确定了有助于理解该家族在 γ-变形菌中的进化位置的分子特征。在本研究中，我们调查了在高度保守的蛋白质中鉴定的插入是否也可以用作分类学分类和鉴定黄单胞菌科成员的分子标记。选择了四种看家蛋白（DNA 修复和复制相关以及蛋白质翻译酶）。插入允许区分该家族内的植物病原和植物生长促进组，并且这些插入的氨基酸序列允许区分不同的属，最终区分物种以及病原体。此外，蛋白质 MutS 和 DNA 聚合酶 III（α 亚基）中的插入在苛养木霉中是保守的，但 DNA 连接酶 NAD 依赖性和 Valyl tRNA 合成酶中的特征区分了该属内的特定亚种。可以根据 MutS、DNA 聚合酶 III（α 亚基）和 Valyl tRNA 合成酶中的插入来区分窄养单胞菌属和假单胞菌属，尽管插入 DNA 连接酶 NAD 依赖性在物种水平上区分这些细菌。所有这些插入区分黄单胞菌内的物种和病原体。因此，