Objectives To evaluate mixotrophic cultivation of microalgae-bacteria consortium in raw wastewater by stepwise addition of fermented effluent containing volatile fatty acids (VFAs). Results Stepwise increase of VFAs enhanced algal biomass and lipid production, ammonia and phosphate removals. The highest biomass and lipid yield were 1.94 g L −1 and 310 mg L −1 when the addition of fermented effluent containing VFAs increased to 30% (v/v). With the same cultivation conditions, the maximum removals efficiency of ammonia and phosphate were 26.4 and 11.3 mg L −1 d −1 . Bacterial diversities increased with the increasing concentration of VFAs and their communities were identified as phyla Actinobacteria, Bacteroidetes, Cyanobacteria and Proteobacteria. Conclusions Although bacterial quantities increased with algae growth concurrently, the objective of culturing microalgae-bacteria consortium in raw wastewater without sterilization to produce biomass and lipid yield still can be realized.

中文翻译：

---

使用挥发性脂肪酸从原废水中的当地小球藻-细菌聚生体连续生产生物量和脂质

目的 通过逐步添加含有挥发性脂肪酸 (VFA) 的发酵废水，评估原废水中微藻-细菌聚生体的混合营养培养。结果 VFA 的逐步增加增强了藻类生物量和脂质生产、氨和磷酸盐的去除。当添加含有 VFA 的发酵流出物增加到 30% (v/v) 时，最高的生物量和脂质产量分别为 1.94 g L -1 和 310 mg L -1。在相同的培养条件下，氨和磷酸盐的最大去除效率分别为26.4和11.3 mg L -1 d -1 。细菌多样性随着 VFA 浓度的增加而增加，它们的群落被确定为放线菌门、拟杆菌门、蓝藻门和变形菌门。结论 虽然细菌数量随着藻类的生长而增加，