The present study evaluated the effect of probiotic Lactobacillus sp. against pathogenic bacteria Vibrio harveyi in freshwater prawn Macrobrachium rosenbergii. The bacteria were isolated from the gut of prawn and identified using biochemical and PCR tests. Inoculation of probiotic (9 log CFU/g) and pathogenic bacteria (5 log CFU/g) mixture was employed for in vitro test. The in vivo challenge test was conducted in glass aquaria (45 × 30 × 30 cm³) and also in an earthen pond (70 m², 1 m depth) for 8 weeks, individually. In both cases, only the treatment group was provided probiotic (9 log CFU/g) incorporated feed. Growth, digestibility, and immune response of prawn were determined. Both in the in vitro and in vivo test, V. harveyi growth was found to be reduced significantly at 8 hr (from 5 to 4 log CFU/g) and after 50 days (from 7 to 6 log CFU/g) of probiotic administration, respectively. In the pond, higher growth and better digestibility were found in the treatment group (WG%, 549. 30 ± 40.02%; SGR%, 3.12 ± 0.10%; amylase, 1.23 unit/mg; protease, 2.47 unit/mg) compared to the control group (WG%, 310.77 ± 18.68%; SGR%, 2. 35 ± 0.08%; 0.7 unit/mg; protease, 1.82 unit/mg). The immune response was found to be significantly different (P < 0.05) only for SGH (control, 22.76 ± 5.54%; probiotic, 19.32 ± 5.13%) and NGH (control, 75.12 ± 17.12%; probiotic, 78.12 ± 10.11%). Overall, the probiotic bacteria enhanced weight gain, digestibility, and immune response in the experimental prawn. Therefore, Lactobacillus sp. could be used as a potential probiotic for prawn culture against bacterial diseases.

本研究评估了益生菌乳杆菌的作用。对淡水虾罗氏沼虾中的哈氏弧菌具有致病性。从虾肠中分离出细菌，并使用生化和PCR测试进行鉴定。益生菌（9 log CFU / g）和致病菌（5 log CFU / g）混合物的接种用于体外测试。在玻璃水族箱（45×30×30 cm ³）和土池（70 m ²）中进行了体内挑战试验，深度1 m），持续8周。在这两种情况下，仅向治疗组提供益生菌（9 log CFU / g）掺入饲料。确定虾的生长，消化率和免疫反应。在体外和体内试验中，发现益生菌施用后8小时（从5到4 log CFU / g）和50天（从7到6 log CFU / g）哈氏弧菌的生长都显着减少。 ， 分别。在池塘中，与之相比，治疗组的生长更高，消化率更高（WG％，549。30±40.02％； SGR％，3.12±0.10％；淀粉酶，1.23单位/毫克；蛋白酶，2.47单位/毫克）对照组（WG％，310.77±18.68％； SGR％，2。35±0.08％； 0.7单位/ mg；蛋白酶，1.82单位/ mg）。发现免疫反应显着不同（P <0.05）仅适用于SGH（对照组，22.76±5.54％;益生菌，19.32±5.13％）和NGH（对照组，75.12±17.12％;益生菌，78.12±10.11％）。总体而言，益生菌可增强实验虾的体重，消化率和免疫反应。因此，乳杆菌属。可以用作对虾培养抗细菌疾病的潜在益生菌。