Maslinic acid is an active member of pentacyclic triterpenes predominantly found in dietary plants including hawthorn berries and olive fruit skins. It has been reported to show immense pharmacological and biological importance including anticancer property. This research was initiated to explore the anticancer potential of maslinic acid against human neuroblastoma. The effects of maslinic acid on cellular apoptosis, ROS generation, cell migration and invasion, caspase activation and targeting MAPK/ERK signaling pathway were investigated. The proliferation percentage was calculated by performing of MTT assay. AO/EB and annexin V/PI staining assays along with western blotting were used to monitor the apoptosis and expressions of apoptosis connected proteins. Spectrofluorometry was used for ROS monitoring. To assess the anti-metastatic effects of maslinic acid on neuroblastoma cells, transwell chambers assays for migration as well as invasion were executed. Western blotting was implemented to establish the expressions of MAPK/ERK signaling pathway connected proteins. Results evidenced remarkable anticancer potential of maslinic acid against human neuroblastoma. It induced dose as well as time reliant anti-proliferative effects against SHSY-5Y cells selectively. The underlying mechanism of cancer suppressive effects of maslinic acid was found to mediate via caspase-dependent apoptosis. Further, ROS production amplified terrifically with exposure of SHSY-5Y to higher maslinic acid doses. Cell migration and invasion in SHSY-5Y cells were both reduced remarkably by maslinic acid. Finally, the activity of proteins associated with MAPK/ERK signaling pathway was found to be significantly reduced with increasing maslinic acid doses. In conclusion, it was observed that maslinic acid possesses a great anti-neuroblastoma potential and could be considered for its chemotherapy provided further investigations are recommended.

山lin酸是五环三萜的活性成员，主要存在于包括山楂浆果和橄榄果皮在内的饮食植物中。据报道显示出巨大的药理和生物学重要性，包括抗癌特性。开始这项研究以探索山楂酸对人神经母细胞瘤的抗癌潜力。研究了山楂酸对细胞凋亡，ROS生成，细胞迁移和侵袭，胱天蛋白酶激活和靶向MAPK / ERK信号通路的影响。通过进行MTT测定来计算增殖百分比。AO / EB和膜联蛋白V / PI染色测定以及Western印迹用于监测细胞凋亡和凋亡相关蛋白的表达。荧光光谱法用于ROS监测。为了评估山楂酸对神经母细胞瘤细胞的抗转移作用，进行了用于迁移和侵袭的transwell小室测定法。进行蛋白质印迹以建立MAPK / ERK信号通路连接蛋白的表达。结果证明山楂酸对人神经母细胞瘤具有显着的抗癌潜力。它选择性地诱导针对SHSY-5Y细胞的剂量和时间依赖性的抗增殖作用。发现山楂酸的癌症抑制作用的潜在机制是通过胱天蛋白酶依赖性凋亡介导的。此外，随着SHSY-5Y暴露于更高的山楂酸剂量，ROS的产生会急剧增加。山楂酸能显着减少SHSY-5Y细胞的迁移和侵袭。最后，发现与MAPK / ERK信号通路相关的蛋白质活性随山楂酸剂量的增加而显着降低。总之，观察到山楂酸具有很大的抗神经母细胞瘤潜能，如果建议进一步研究，可以考虑将其用于化学疗法。