Abstract

Several newly synthesized or forgotten silica-based stationary phases proposed for liquid chromatography are described, including non-endcapped, short-chain alkyl phases; hydrophilic and polar-endcapped stationary phases; polar-embedded alkyl phases; long-chain alkyl phases. Stationary phases with aromatic, cyanopropyl, diol and aminopropyl functionalities are also reviewed. Stationary phases of particular interest are biomolecular materials – based on immobilized cholesterol, aminoacids, peptides, proteins or lipoproteins. Packing materials involving macrocyclic chemistry (crown ethers; calixarenes; aza-macrocycles; oligo-and polysaccharides including these of marine origin - chitin- or chitosan-based; macrocyclic antibiotics) are discussed. Since many stationary phases developed for one type of applications (e.g. chiral separation) have been found useful in solving other analytical problems (e.g. drug’s plasma protein binding ability), it seemed reasonable to discuss particular chemistries behind the stationary phases presented in this review rather than specific types of interactions or chromatographic modes.

摘要

介绍了用于液相色谱的几种新合成或被遗忘的硅胶基固定相，包括未封端的短链烷基固定相；亲水性和极性封端的固定相；极性嵌入的烷基相；长链烷基相。还回顾了具有芳香族、氰丙基、二醇和氨基丙基官能团的固定相。特别感兴趣的固定相是生物分子材料——基于固定化的胆固醇、氨基酸、肽、蛋白质或脂蛋白。讨论了涉及大环化学的包装材料（冠醚；杯芳烃；氮杂大环；寡糖和多糖，包括海洋来源的这些 - 甲壳素或壳聚糖；大环抗生素）。由于为一种类型的应用开发了许多固定相（例如