Abstract Objective To evaluate the therapeutic effect of platelet lysate (PL)/vancomycin/nano-hydroxyapatite sustained-release material on treating staphylococcus aureus-induced infectious bone defects. Methods 40 New Zealand white rabbits were inoculated with staphylococcus aureus to construct the chronic tibial infectious bone defect model. After incision, debridement and washing, control group 1 was not given any filling, control group 2 was filled with PL/nano-hydroxyapatite sustained release material, control group 3 was filled with vancomycin/ nano-hydroxyapatite sustained release material, and the treatment group was filled with PL/vancomycin/nano-hydroxyapatite sustained-release material. Afterwards, the drug release profiles were determined in vitro and in vivo. Then, X-rays and bone specimens were used to evaluate the efficacy of the treatments. Results TGF-β and PDGF were effectively released for 28 days in vitro. In addition, results of the inhibition zone experiment of the composite material proved that vancomycin had favorable antibacterial activity, which effectively suppressed bacteria for as long as 43 days, thus achieving the sustained-release antibacterial effect. The drug release profiles in vitro also demonstrated that the vancomycin concentration within the lesion region was the highest in composite material, and the infection in experimental rabbits was markedly alleviated. The original backbone deformity regained the normal shape, the normal bone marrow structure began to recover 6 weeks later, and the nano-hydroxyapatite transformed into the trabecula structure. By contrast, the inflammation in the control group still existed, with no obvious new bone formation. Conclusion The PL/vancomycin/nano-hydroxyapatite sustained-release material effectively treats chronic infectious bone defects.

中文翻译：

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PL/万古霉素/纳米羟基磷灰石缓释材料治疗感染性骨缺损

摘要 目的评价血小板裂解物（PL）/万古霉素/纳米羟基磷灰石缓释材料治疗金黄色葡萄球菌感染性骨缺损的疗效。方法40只新西兰大白兔接种金黄色葡萄球菌，构建慢性胫骨感染性骨缺损模型。切开、清创、冲洗后，对照组1不做任何填充，对照组2填充PL/纳米羟基磷灰石缓释材料，对照组3填充万古霉素/纳米羟基磷灰石缓释材料，治疗组填充有 PL/万古霉素/纳米羟基磷灰石缓释材料。然后，在体外和体内确定药物释放曲线。然后，X射线和骨标本用于评估治疗效果。结果 TGF-β和PDGF在体外有效释放28天。此外，复合材料抑菌圈实验结果证明万古霉素具有良好的抗菌活性，可有效抑制细菌长达43天，达到缓释抑菌效果。体外药物释放曲线也表明，复合材料病变区域内的万古霉素浓度最高，实验兔的感染明显减轻。原有骨干畸形恢复正常形态，6周后正常骨髓结构开始恢复，纳米羟基磷灰石转变为小梁结构。相比之下，对照组炎症依然存在，无明显新骨形成。结论 PL/万古霉素/纳米羟基磷灰石缓释材料可有效治疗慢性感染性骨缺损。