Abstract Bone regeneration after trauma, pathologic and surgical procedures is considered a major medical challenge. Due to limitations in using conventional approaches, cell based regenerative strategies may provide an alternative option to address such issues. In the current study, we sought to determine the osteogenic potential of dental pulp stem cells (DPSCs) isolated from impacted 3rd molars. DPSCs were isolated from human dental pulp tissue (n=6) using explant culture. Growth characteristics of DPSCs were determined using plating efficiency, and the number and time of population doublings. After characterization, DPSCs were induced to differentiate into osteoblasts and were assessed using polymerase chain reactions (PCR) and histological analysis. Results indicated that DPSCs can be isolated from impacted human third molars, and that DPSCs exhibited typical fibroblastic morphology and excellent proliferative potential. In addition, morphological changes, histological analysis and expression of lineage specific genes confirmed osteogenic differentiation of DPSCs. In conclusion, DPSCs isolated from impacted 3rd molars have high proliferative potential and ability to differentiate into osteoblasts.

中文翻译：

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人牙髓干细胞表现出成骨分化潜能

摘要 创伤、病理和外科手术后的骨再生被认为是一项重大的医学挑战。由于使用传统方法的限制，基于细胞的再生策略可以提供解决此类问题的替代选择。在目前的研究中，我们试图确定从受影响的第三磨牙中分离的牙髓干细胞 (DPSC) 的成骨潜力。使用外植体培养从人牙髓组织 (n=6) 中分离出 DPSC。DPSCs 的生长特性是通过电镀效率以及种群倍增的次数和时间来确定的。表征后，DPSCs 被诱导分化为成骨细胞，并使用聚合酶链反应 (PCR) 和组织学分析进行评估。结果表明 DPSCs 可以从受影响的人类第三磨牙中分离出来，DPSCs 表现出典型的成纤维细胞形态和极好的增殖潜力。此外，形态学变化、组织学分析和谱系特异性基因的表达证实了 DPSCs 的成骨分化。总之，从受影响的第三磨牙中分离的 DPSCs 具有高增殖潜力和分化成成骨细胞的能力。