Abstract Fisetin is a polyphenolic compound with anti-inflammatory and antioxidant properties. Inflammation and reactive oxygen species play a major role in the pathophysiology of the dry eye syndrome (DES). Patients with DES undergo symptomatic treatment using eye drops known as artificial tears. Addition of fisetin into the eye drops could result in a better recovery of the eye surface. This experimental study examines the stability of fisetin in selected eye drops (Arufil, Hypromelóza-P, Ocutein, Refresh). Absorption spectra of fisetin were measured in selected eye drops, dimethylsulphoxide (DMSO), deionized water and normal saline solution (NSS) during a period of four weeks. The fisetin absorption maximum was placed at 350 – 390 nm depending on the solvent. Good stability of fisetin solutions were observed in DMSO and deionized water. The highest stability of fisetin in selected eye drops was observed in Hypromelóza-P. Irreversible fisetin structural changes were detected in Arufil, Ocutein, Refresh and NSS. For further clinical evaluation, fisetin solution in Hypromelóza-P could be examined. Graphical Abstract

中文翻译：

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滴眼液中天然多酚漆黄素的稳定性 滴眼液中漆黄素的稳定性

摘要 鱼腥草素是一种具有抗炎和抗氧化特性的多酚化合物。炎症和活性氧在干眼症 (DES) 的病理生理学中起主要作用。DES 患者使用称为人工泪液的滴眼液进行对症治疗。在滴眼液中加入非瑟酮可以使眼表更好地恢复。该实验研究检查了非瑟酮在选定滴眼液（Arufil、Hypromelóza-P、Ocutein、Refresh）中的稳定性。在选定的滴眼液、二甲基亚砜 (DMSO)、去离子水和生理盐水 (NSS) 中，在 4 周内测量了非瑟酮的吸收光谱。视溶剂而定，漆黄吸收最大值位于 350 – 390 nm。在 DMSO 和去离子水中观察到非瑟酮溶液具有良好的稳定性。在 Hypromelóza-P 中观察到非瑟酮在选定滴眼液中的最高稳定性。在 Arufil、Ocutein、Refresh 和 NSS 中检测到不可逆的漆黄素结构变化。为了进一步的临床评估，可以检查 Hypromelóza-P 中的非瑟酮溶液。图形概要