Abstract A unique method was used to synthesize extremely stable silver stearate nanoparticles (AgStNPs) incorporated in an organic-based monolith. The facile strategy was then used to selectively isolate hemeproteins, myoglobin (Myo) and hemoglobin (Hb). Ethyl alcohol, silver nitrate, and stearic acid were, respectively, utilized as reducing agents, silver precursors, and capping agents. The color changed to cloudy from transparent, indicating that AgStNPs had been formed. AgStNP nanostructures were then distinctly integrated into the natural polymeric scaffold. To characterize the AgStNP–methacrylate polymeric monolith and the silver nanoparticles, energy-dispersive X-ray (EDX), scanning electron microscopy (SEM), and Fourier-transform infrared (FT-IR) spectroscopy were used. The results of the SEM analysis indicated that the AgStNP–methacrylate polymeric monolith’s texture was so rough in comparison with that of the methacrylate polymeric monolith, indicating that the extraction process of the monolith materials would be more efficient because of the extended surface area of the absorbent. The comparison between the FT-IR spectra of AgStNPs, the bare organic monolith, and AgStNP–methacrylate polymeric monolith confirms that the AgStNPs were immobilized on the surface of the organic monolith. The EDX profile of the built materials indicated an advanced peak of the Ag sequence which represented an Ag atom of 3.27%. The results therefore established that the AgStNPs had been successfully integrated into the monolithic materials. Extraction efficiencies of 92% and 97% were used to, respectively, recover preconcentrated Myo and Hb. An uncomplicated method is a unique approach of both fabrication and utilization of the nanosorbent to selectively isolate hemeproteins. The process can further be implemented by using other noble metals.

中文翻译：

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将硬脂酸银纳米颗粒掺入甲基丙烯酸酯聚合物整料中以分离血红蛋白

摘要 一种独特的方法被用来合成结合在有机基整料中的极其稳定的硬脂酸银纳米颗粒 (AgStNPs)。然后使用简便的策略选择性地分离血红素蛋白、肌红蛋白 (Myo) 和血红蛋白 (Hb)。乙醇、硝酸银和硬脂酸分别用作还原剂、银前体和封端剂。颜色从透明变为混浊，表明已形成 AgStNPs。然后将 AgStNP 纳米结构明显地整合到天然聚合物支架中。为了表征 AgStNP-甲基丙烯酸酯聚合物整料和银纳米颗粒，使用了能量色散 X 射线 (EDX)、扫描电子显微镜 (SEM) 和傅里叶变换红外 (FT-IR) 光谱。SEM 分析结果表明，AgStNP-甲基丙烯酸酯聚合物整料的质地与甲基丙烯酸酯聚合物整料相比非常粗糙，表明由于吸收剂的表面积扩大，整料的提取过程将更有效。 . AgStNPs、裸有机整料和 AgStNP-甲基丙烯酸酯聚合物整料的 FT-IR 光谱之间的比较证实，AgStNPs 固定在有机整料的表面。构建材料的 EDX 曲线表明 Ag 序列的先进峰代表 3.27% 的 Ag 原子。因此，结果表明 AgStNPs 已成功集成到整体材料中。分别使用 92% 和 97% 的提取效率，恢复预浓缩的 Myo 和 Hb。一种简单的方法是制造和利用纳米吸附剂选择性分离血红素蛋白的独特方法。该工艺还可以通过使用其他贵金属来实现。