Abstract The microalga Dunaliella has been the focus of attention over recent decades owing to its high biotechnological potential for the production of β-carotene, biofuels and even as a good expression system for the production of recombinant proteins. Different species of this genus have unique features, biological characteristics and biotechnological potential. Therefore, it is necessary to have a clear and reliable taxonomic method to identify different species of Dunaliella. Although several taxonomic systems are available for Dunaliella based on morphological, physiological and molecular features, none of these methods are reliable enough and some controversies exist over different classification systems. In the current study, molecular techniques and bioinformatics tools have been used to re-assess the phylogenetic position of Dunaliella species based on 18S ribosomal DNA (18S rDNA), ITS and rbcL regions. The overall findings based on these markers provide a new and more reliable tool for phylogenetic analysis of Dunaliella species/strains.

中文翻译：

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使用 18S 核糖体 DNA、ITS 和 rbcL 分子标记研究伊朗样本中的杜氏藻属（杜氏藻科）：一种系统发育方法

摘要 杜氏微藻因其在生产 β-胡萝卜素、生物燃料方面的高生物技术潜力，甚至作为生产重组蛋白的良好表达系统而成为近几十年来关注的焦点。该属的不同物种具有独特的特征、生物学特性和生物技术潜力。因此，需要有一个清晰可靠的分类方法来鉴别不同种类的杜氏藻。尽管基于形态学、生理学和分子特征的杜氏藻有多种分类系统可用，但这些方法都不够可靠，并且在不同的分类系统上存在一些争议。在目前的研究中，分子技术和生物信息学工具已被用于基于 18S 核糖体 DNA (18S rDNA)、ITS 和 rbcL 区域重新评估杜氏藻属物种的系统发育位置。基于这些标记的总体发现为杜氏藻属物种/菌株的系统发育分析提供了一种新的、更可靠的工具。