Abstract Introduction Toll-like receptors (TLRs) play an important role in fast activation of the immune response to a variety of pathogens, including parasites. In this study, we focused on TLR2, because this receptor is one of the best known and most frequently analysed members of the TLR family. The aim of this study was to assess the effect of Trichinella spiralis on expression of TLR2 during the intestinal stage of infection. Material and Methods The experimental material consisted of isolates prepared from the intestines (jejunum and colon) of BALB/c mice infected with T. spiralis taken at 4, 8, and 16 days post infection. Results Our results based on quantitative real-time PCR showed that the mRNA level for TLR2 was statistically significantly higher in the jejuna of mice infected with T. spiralis than in this tissue of uninfected mice. In addition, the presence of TLR2 protein in the intestinal phase of trichinellosis was confirmed by a strong positive immunohistochemical reaction. Conclusion Our results indicate that infection with T. spiralis changes the expression of TLR2 in the small intestine of the mouse host and suggest a contribution of these receptors to the host defence mechanisms during experimental trichinellosis.

中文翻译：

---

旋毛虫病肠期TLR2表达的变化

摘要 简介 Toll 样受体 (TLR) 在快速激活对多种病原体（包括寄生虫）的免疫反应中起重要作用。在这项研究中，我们专注于 TLR2，因为这种受体是 TLR 家族中最著名和最常被分析的成员之一。本研究的目的是评估旋毛虫在肠道感染阶段对 TLR2 表达的影响。材料和方法 实验材料由从感染 T.spiralis 的 BALB/c 小鼠的肠道（空肠和结肠）制备的分离物组成，这些小鼠在感染后 4、8 和 16 天采集。结果 我们基于定量实时 PCR 的结果表明，TLR2 的 mRNA 水平在感染 T.spiralis 的小鼠的空肠中显着高于未感染小鼠的该组织。此外，通过强阳性免疫组织化学反应证实了旋毛虫病肠期TLR2蛋白的存在。结论 我们的结果表明，感染 T.spiralis 会改变小鼠宿主小肠中 TLR2 的表达，并表明这些受体在实验性旋毛虫病期间对宿主防御机制的贡献。