Abstract Introduction Cysticercosis caused by the larval stage of Taenia hydatigena is economically the most important endemic parasitic disease in Iraq. Few data are available relating to the genetic divergence of this helminth. This study aimed to molecularly characterise Cysticercus tenuicollis isolates from sheep in Sulaymaniyah province, Iraq. Material and Methods DNA extraction and amplification of specimens of C. tenuicollis from 46 sheep were conducted by PCR for the mitochondrial 12S rRNA gene. The 19 amplicons were subjected to purification and partial sequencing. Results Five 12S rRNA nucleotide sequence haplotypes were found. The pairwise nucleotide difference between haplotypes of 12S rRNA gene ranged from 0.2% to 0.7%. Four out of the five haplotypes of C. tenuicollis contained one to two base mutations and were discovered in Iraq for the first time, and this may be a unique mutation globally which has not been recorded previously. Three newly recorded haplotypes contained only one single mutation, and the other one contained two mutations. Phylogenetic analysis showed that all isolated strains were closely related to Iranian sheep isolates. Conclusions Four new strains of T. hydatigena were discovered for the first time in the study area.

中文翻译：

---

伊拉克苏莱曼尼亚省屠宰羊的细颈囊尾蚴分子研究

摘要 引言 由紫绦虫幼虫期引起的囊尾蚴病是伊拉克经济上最重要的地方性寄生虫病。很少有与这种蠕虫的遗传差异有关的数据。本研究旨在对伊拉克苏莱曼尼亚省绵羊的细尾囊尾蚴分离物进行分子表征。材料与方法 对 46 只绵羊的细颈梭菌标本进行 DNA 提取和扩增，采用 PCR 方法检测线粒体 12S rRNA 基因。对 19 个扩增子进行纯化和部分测序。结果共发现5个12S rRNA核苷酸序列单倍型。12S rRNA基因单倍型之间的成对核苷酸差异范围为0.2%至0.7%。C的五个单倍型中的四个。tenuicollis 含有一到两个碱基突变，首次在伊拉克发现，这可能是全球独一无二的突变，以前没有记录。三个新记录的单倍型仅包含一个单一突变，另一个包含两个突变。系统发育分析表明，所有分离株均与伊朗绵羊分离株密切相关。结论 研究区首次发现了4个新菌株T. hydatigena。