Oil reservoirs contain large amounts of hydrocarbon rich produced water, trapped in underground channels. Focus of this study was isolation of PHA producers from produced water concomitant with optimization of production using animal fat and glycerol as carbon source. Bacterial strains were identified as Bacillus subtilis (PWA), Pseudomonas aeruginosa (PWC), Bacillus tequilensis (PWF), and Bacillus safensis (PWG) based on 16S rRNA gene sequencing. Similar amounts of PHA were obtained using animal fat and glycerol in comparison to glucose. After 24 h, high PHA production on glycerol and animal fat was shown by strain PWC (5.2 g/ L, 6.9 g/ L) and strain PWF (12.4 g/ L, 14.2 g/ L) among all test strains. FTIR analysis of PHA showed 3-hydroxybutyrate units. The capability to produce PHA in the strains was corroborated by PhaC synthase gene sequencing. Focus of future studies can be the use of lipids and glycerol on industrial scale.

中文翻译：

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产生的水细菌将动物脂肪和甘油生物转化为聚羟基链烷酸酯

油藏中有大量富含烃的采出水，被困在地下通道中。这项研究的重点是从采出水中分离出PHA生产者，并使用动物脂肪和甘油作为碳源优化生产。细菌菌株被鉴定为枯草芽孢杆菌（PWA），铜绿假单胞菌（PWC），龙舌兰芽孢杆菌（PWF）和安全芽孢杆菌（PWG）基于16S rRNA基因测序。与葡萄糖相比，使用动物脂肪和甘油可获得相似量的PHA。24小时后，所有测试菌株中的PWC菌株（5.2 g / L，6.9 g / L）和PWF菌株（12.4 g / L，14.2 g / L）显示出甘油和动物脂肪上PHA的高产量。PHA的FTIR分析显示3-羟基丁酸酯单元。通过PhaC合酶基因测序证实了在菌株中产生PHA的能力。未来研究的重点可能是在工业规模上使用脂质和甘油。