Abstract The aim of the study was to determine whether feeding rats a diet without added Cu increases oxidation of macromolecules in tissues, as well as epigenetic changes in the brain. The rats were divided into two groups: the Cu-6.5 group which was fed a diet with a standard content of Cu in mineral mixture – 6.5 mg Cu from CuCO3 per kg of diet; and the Cu-0 group which was fed a diet with a mineral mix without Cu supplementation. At the end of the experiment the rats were weighed and blood samples were collected. Finally, the rats were euthanized and then the liver, small intestine, spleen, kidneys, heart, brain, lung, testes and leg muscles were removed and weighed. In the blood of Cu-0 rats the lower Cp activity and greater GPx and CAT activity than in Cu-6.5 rats were noticed. In the liver, lungs, heart and testes of Cu-0 rats, a decreased content of Cu were noticed. Application of Cu-0 diets resulted in increased LOOH level in the small intestine, liver, and heart, as well as increased MDA content in the liver, spleen, lungs, brain and testes. The Cu-0 treatment caused a decrease in SOD activity in the heart, lungs and testes of the rats and a decrease in CAT activity in the small intestine. In the brain and testes of rats from the Cu-0 treatment, lower content of GSH + GSSG was observed. The brain of rats from the Cu-0 treatment showed an increase in the level of PCs, 8-OHdG, Casp 8 and DNA methylation. The research has shown that a deficiency of Cu in the diet impairs the body’s antioxidant defences, which in turn leads to increased lipid oxidation in the liver, small intestinal wall, heart, spleen, lungs, brain and testes, as well as to oxidation of proteins and DNA in the brain. A deficiency of Cu in the diet also increases methylation of cytosine in the brain.

中文翻译：

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不添加铜的大鼠饮食对组织中氧化还原状态和大脑表观遗传变化的影响

摘要 本研究的目的是确定给大鼠喂食不添加铜的饮食是否会增加组织中大分子的氧化以及大脑中的表观遗传变化。将大鼠分为两组：Cu-6.5 组，饲喂矿物混合物中含有标准铜含量的饮食 - 每公斤饮食中含有 6.5 毫克 CuCO3 的铜；和 Cu-0 组，其饮食中含有矿物质混合物，但不补充铜。在实验结束时，对大鼠称重并收集血样。最后，将大鼠安乐死，然后取出肝脏、小肠、脾脏、肾脏、心脏、大脑、肺、睾丸和腿部肌肉并称重。在 Cu-0 大鼠的血液中，观察到比 Cu-6.5 大鼠更低的 Cp 活性和更高的 GPx 和 CAT 活性。在 Cu-0 大鼠的肝、肺、心脏和睾丸中，注意到Cu含量降低。Cu-0 饮食的应用导致小肠、肝脏和心脏中 LOOH 水平增加，以及肝脏、脾脏、肺、大脑和睾丸中 MDA 含量增加。Cu-0 处理导致大鼠心脏、肺和睾丸中 SOD 活性的降低以及小肠中 CAT 活性的降低。在来自 Cu-0 治疗的大鼠的大脑和睾丸中，观察到较低的 GSH + GSSG 含量。Cu-0 治疗大鼠的大脑显示 PC、8-OHdG、Casp 8 和 DNA 甲基化水平增加。研究表明，饮食中缺乏铜会损害人体的抗氧化防御，进而导致肝脏、小肠壁、心脏、脾脏、肺、大脑和睾丸中脂质氧化增加，以及大脑中蛋白质和DNA的氧化。饮食中铜的缺乏也会增加大脑中胞嘧啶的甲基化。