The study investigated egg quality aspects such as astaxanthin concentration, E/Z-isomer ratio, and yolk color in laying hens fed with astaxanthin-containing diets. Dried Paracoccus carotinifaciens cell powder (Panaferd-AX) and fine cell powder (Panaferd-P) were used as sources of astaxanthin, with average particle diameters of approximately 100 μm and 10 μm, respectively. Paracoccus carotinifaciens contains valuable rare carotenoids such as adonirubin and adonixanthin, and thus the concentrations of these carotenoids in egg yolk were also evaluated. The E/Z-isomer ratios of the egg yolk carotenoids were determined by normal-phase high-performance liquid chromatography (HPLC) with an improved solvent system. Feeding diets containing P. carotinifaciens resulted in increased concentrations of astaxanthin, adonirubin, and adonixanthin in egg yolk, as well as a marked increase in the yolk color fan score; values associated with the Panaferd-P-containing diet were higher than those associated with the Panaferd-AX-containing diet. For example, the astaxanthin concentration in egg yolks of hens fed with the Panaferd-AX- and Panaferd-P-containing diets for 21 days were 1.21 μg/g and 1.85 μg/g, respectively. This indicates that the pulverization treatment of the P. carotinifaciens powder increased the efficiency of carotenoid accumulation in the egg yolk. Moreover, more than 95% of astaxanthin in P. carotinifaciens was present as the all-E-isomer. However, approximately 25% of astaxanthin in egg yolk was present as the Z-isomers. In recent years, astaxanthin Z-isomers have attracted substantial attention as they exhibit a greater bioavailability and bioactivity than the all-E-isomer. These data are important not only for understanding egg yolk pigmentation but also for improving the nutritional value of hens’ egg yolk through the addition of P. carotinifaciens to their diet.

中文翻译：

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使用来自类胡萝卜素副球菌的富含虾青素的干细胞粉改善蛋鸡蛋黄色素沉着的可能性

该研究调查了用含虾青素日粮喂养的蛋鸡的鸡蛋质量方面，例如虾青素浓度、E/Z 异构体比率和蛋黄颜色。干燥的副球菌类胡萝卜素细胞粉末 (Panaferd-AX) 和细细胞粉末 (Panaferd-P) 用作虾青素的来源，平均粒径分别约为 100 μm 和 10 μm。Paracoccus carotinifaciens 含有珍贵的稀有类胡萝卜素，如阿多红素和阿多黄素，因此还评估了这些类胡萝卜素在蛋黄中的浓度。蛋黄类胡萝卜素的 E/Z 异构体比率通过正相高效液相色谱 (HPLC) 与改进的溶剂系统进行测定。饲喂含有胡萝卜素假单胞菌的日粮导致蛋黄中虾青素、腺红素和腺黄素的浓度增加，以及蛋黄颜色粉丝评分的显着增加；与含有 Panaferd-P 的饮食相关的值高于与含有 Panaferd-AX 的饮食相关的值。例如，用含有 Panaferd-AX 和 Panaferd-P 的饮食喂养 21 天的母鸡的蛋黄中虾青素浓度分别为 1.21 μg/g 和 1.85 μg/g。这表明P.carotinifaciens粉末的粉碎处理提高了蛋黄中类胡萝卜素积累的效率。此外，P. carotinifaciens 中超过 95% 的虾青素以全 E 异构体的形式存在。然而，蛋黄中大约 25% 的虾青素以 Z 异构体的形式存在。近年来，虾青素 Z 异构体由于比全 E 异构体具有更高的生物利用度和生物活性而引起了广泛关注。