In a world where an eco-friendlier approach is becoming more and more necessary, it is essential to reduce waste production and to reuse residues of the company’s supply chain. Coffee silverskin (CS) and spent coffee ground (SCG), two by-products of coffee production, are important sources of bioactive compounds and, for this, some authors have proposed their reuse in the nutraceutical, food, and cosmetic sector. However, their potential enzyme inhibitory properties have been poorly investigated. Hence, the objective of the current work was to study the enzymatic inhibitory activities against acetylcholinesterase, butyrylcholinesterase, α-amylase, α-glucosidase, and tyrosinase of different extracts of CS and SCG. Before these in vitro bioassays, the phytochemical composition of each extract was investigated via colorimetric assays and HPLC-MS/MS analysis. In addition, the antioxidant activities were evaluated by different chemical approaches. SCG extracts contained a higher content of bioactive compounds, notably the SCG EtOH:H₂O extract was the richest in caffeine and possessed the highest antioxidant activities. The hydroalcoholic and methanolic extracts were shown to be the most active against all tested enzymes, while the water extracts displayed lower activity. Our results showed a weak correlation between bioactive compounds and enzyme inhibitory effects, proving inhibitory activities likely due to non-phenolic molecules such as alkaloids and terpenoids. Obtained findings could be a starting point to develop novel nutraceuticals from CS and SCG.

中文翻译：

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从废咖啡渣和咖啡银皮中获得的不同提取物的化学组成，抗氧化和酶抑制特性。

在一个越来越需要生态友好的方法的世界中，至关重要的是减少废物的产生并重新利用公司供应链中的残留物。咖啡生产中的两种副产品咖啡银皮（CS）和废咖啡渣（SCG）是生物活性化合物的重要来源，为此，一些作者建议将其在营养保健品，食品和化妆品行业中重复使用。但是，对其潜在的酶抑制特性的研究很少。因此，当前工作的目的是研究对CS和SCG不同提取物的乙酰胆碱酯酶，丁酰胆碱酯酶，α-淀粉酶，α-葡萄糖苷酶和酪氨酸酶的酶抑制活性。在进行这些体外生物测定之前，通过比色分析和HPLC-MS / MS分析研究了每种提取物的植物化学组成。另外，通过不同的化学方法评估抗氧化活性。SCG提取物包含较高含量的生物活性化合物，尤其是SCG EtOH：H₂ O提取物是最丰富的咖啡因，具有最高的抗氧化活性。水醇和甲醇提取物显示出对所有测试酶最有活性，而水提取物显示出较低的活性。我们的结果表明生物活性化合物与酶抑制作用之间的相关性较弱，证明抑制活性可能是由于非酚类分子（如生物碱和萜类化合物）引起的。获得的发现可能是从CS和SCG开发新型保健食品的起点。