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Transcriptome analysis of ovarian maturation in a chondrostei Chinese sturgeon Acipenser sinensis.
Journal of Experimental Zoology-B: Molecular and Developmental Evolution ( IF 1.8 ) Pub Date : 2020-06-01 , DOI: 10.1002/jez.b.22973 Shan He 1 , Hao Du 2 , Ping Ren 1 , Xiao-Qian Leng 2 , Qing-Song Tan 1 , Chuang-Ju Li 2 , Xu-Fang Liang 1 , Qi-Wei Wei 2
Journal of Experimental Zoology-B: Molecular and Developmental Evolution ( IF 1.8 ) Pub Date : 2020-06-01 , DOI: 10.1002/jez.b.22973 Shan He 1 , Hao Du 2 , Ping Ren 1 , Xiao-Qian Leng 2 , Qing-Song Tan 1 , Chuang-Ju Li 2 , Xu-Fang Liang 1 , Qi-Wei Wei 2
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Chinese sturgeon (Acipenser sinensis) with an evolutionary history of over 200 million years, has a long lifespan, and an extremely late and asynchronous sexual maturation (8–18 years for males and 14–26 years for females), resulting in the difficulty of mature adult culture. However, little is known about the regulatory mechanisms of the transition among ovarian maturation stages in the Chinese sturgeon. We performed de novo transcriptome sequencing of the Chinese sturgeon at different ovarian maturation stages (FII, FIII, and FIV). The number of differentially expressed genes (DEGs) between FII and FIII/FIV (33,517/34,217) was more than that between FIII and FIV (22,123), suggesting that the transition from FII to FIII/FIV is more important than that from FIII to FIV for ovarian maturation. The number of upregulated genes was more than that of the downregulated genes, suggesting that increased gene expression was involved in the transition from FII to FIII/FIV. The representative pathways of DEGs were steroid biosynthesis, fatty acid biosynthesis, fatty acid elongation, glycerolipid metabolism, biosynthesis of unsaturated fatty acid, and α‐linolenic acid metabolism. The differential expressions from the transcriptome sequencing were validated with real‐time reverse‐transcription polymerase chain reaction. We also found 13 genes in sexual development, female sex determination, gonadal development, ovarian maturation, ovarian follicle development, and oocyte development pathways, which were differently expressed among fish at FII, FIII, and FIV. We suggest that enhanced synthesis of steroid, unsaturated fatty acid, and α‐linolenic acid may contribute to ovarian maturation of the Chinese sturgeon. These potential determinants provide a glimpse of the molecular architecture of ovary development in sturgeons.
中文翻译：
中华Chinese鱼中华cho卵巢成熟的转录组分析
中华st（中华cip）具有超过2亿年的进化历史，寿命长，并且性成熟时间极晚且不同步（男性为8–18岁，女性为14–26岁），这导致成人文化难以成熟。然而，关于中华st在卵巢成熟阶段间过渡的调控机制知之甚少。我们在不同的卵巢成熟阶段（FII，FIII和FIV）对中华st进行了从头转录组测序。FII和FIII / FIV之间的差异表达基因（DEG）的数量（33,517 / 34,217）比FIII和FIV之间的差异表达基因（22,123）的数量更多，这表明从FII到FIII / FIV的过渡比从FIII到FIII的过渡更重要。卵巢成熟的FIV。上调基因的数量多于下调基因的数量，提示增加的基因表达参与了从FII到FIII / FIV的转变。DEGs的代表性途径是类固醇生物合成，脂肪酸生物合成，脂肪酸延伸，甘油脂代谢，不饱和脂肪酸生物合成和α-亚麻酸代谢。通过实时逆转录聚合酶链反应验证了转录组测序的差异表达。我们还在性发育，女性性别决定，性腺发育，卵巢成熟，卵泡发育和卵母细胞发育途径中发现了13个基因，它们在FII，FIII和FIV中的鱼中表达不同。我们建议增加类固醇，不饱和脂肪酸和α-亚麻酸的合成可能有助于中华st的卵巢成熟。
更新日期：2020-06-01
中文翻译：
中华Chinese鱼中华cho卵巢成熟的转录组分析
中华st（中华cip）具有超过2亿年的进化历史，寿命长，并且性成熟时间极晚且不同步（男性为8–18岁，女性为14–26岁），这导致成人文化难以成熟。然而，关于中华st在卵巢成熟阶段间过渡的调控机制知之甚少。我们在不同的卵巢成熟阶段（FII，FIII和FIV）对中华st进行了从头转录组测序。FII和FIII / FIV之间的差异表达基因（DEG）的数量（33,517 / 34,217）比FIII和FIV之间的差异表达基因（22,123）的数量更多，这表明从FII到FIII / FIV的过渡比从FIII到FIII的过渡更重要。卵巢成熟的FIV。上调基因的数量多于下调基因的数量，提示增加的基因表达参与了从FII到FIII / FIV的转变。DEGs的代表性途径是类固醇生物合成，脂肪酸生物合成，脂肪酸延伸，甘油脂代谢，不饱和脂肪酸生物合成和α-亚麻酸代谢。通过实时逆转录聚合酶链反应验证了转录组测序的差异表达。我们还在性发育，女性性别决定，性腺发育，卵巢成熟，卵泡发育和卵母细胞发育途径中发现了13个基因，它们在FII，FIII和FIV中的鱼中表达不同。我们建议增加类固醇，不饱和脂肪酸和α-亚麻酸的合成可能有助于中华st的卵巢成熟。
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