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SIZ1-Mediated SUMO Modification of SEUSS Regulates Photomorphogenesis in Arabidopsis
Plant Communications ( IF 9.4 ) Pub Date : 2020-06-02 , DOI: 10.1016/j.xplc.2020.100080
Xinyu Zhang 1, 2 , Junling Huai 1 , Shuangrong Liu 1, 2 , Jing Bo Jin 2, 3 , Rongcheng Lin 1, 2
Affiliation  

Small ubiquitin-like modifier (SUMO) post-translational modification (SUMOylation) plays essential roles in regulating various biological processes; however, its function and regulation in the plant light signaling pathway are largely unknown. SEUSS (SEU) is a transcriptional co-regulator that integrates light and temperature signaling pathways, thereby regulating plant growth and development in Arabidopsis thaliana. Here, we show that SEU is a substrate of SUMO1, and that substitution of four conserved lysine residues disrupts the SUMOylation of SEU, impairs its function in photo- and thermomorphogenesis, and enhances its interaction with PHYTOCHROME-INTERACTING FACTOR 4 transcription factors. Furthermore, the SUMO E3 ligase SIZ1 interacts with SEU and regulates its SUMOylation. Moreover, SEU directly interacts with phytochrome B photoreceptors, and the SUMOylation and stability of SEU are activated by light. Our study reveals a novel post-translational modification mechanism of SEU in which light regulates plant growth and development through SUMOylation-mediated protein stability.



中文翻译:

SIZ1 介导的 SEUSS SUMO 修饰调节拟南芥的光形态发生

小泛素样修饰剂 (SUMO) 翻译后修饰 (SUMOylation) 在调节各种生物过程中起着至关重要的作用;然而,其在植物光信号通路中的功能和调控在很大程度上是未知的。SEUSS (SEU) 是一种转录共调节剂,可整合光和温度信号通路,从而调节拟南芥植物的生长发育. 在这里,我们表明 SEU 是 SUMO1 的底物,替换四个保守的赖氨酸残基会破坏 SEU 的 SUMO 化,削弱其在光和热形态发生中的功能,并增强其与光致变色相互作用因子 4 转录因子的相互作用。此外,SUMO E3 连接酶 SIZ1 与 SEU 相互作用并调节其 SUMO 化。此外,SEU 直接与光敏色素 B 光感受器相互作用,SEU 的 SUMO 化和稳定性被光激活。我们的研究揭示了一种新的 SEU 翻译后修饰机制,其中光通过 SUMOylation 介导的蛋白质稳定性来调节植物的生长和发育。

更新日期:2020-06-02
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