Abstract We developed an automatic cell culture platform to facilitate the research and development of stem cell differentiation. This platform consists of a distributor of culture media containing different cell factors, a culture unit allowing either static or dynamic exchange of the medium and a central unit for pumping and process control, respectively. This platform was firstly used for cell culture with a tissue culture dish at a high medium exchange rate, showing an increased cell growth due to enhanced uptake of the nutrients and enhanced clearance of the metabolites. Then, the platform was used for automatic differentiation of human induced pluripotent stem cells (hiPSCs) toward cardiomyocytes which involved six types of culture media. As results, functional cardiomyocytes could be obtained after automatic processing during two weeks, showing regular beating over a large area. Details are described to promote the application of the platform to other types of stem cell differentiation or cell-based assays.

中文翻译：

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用于分化人诱导多能干细胞的自动细胞培养平台

摘要 我们开发了一个自动细胞培养平台，以促进干细胞分化的研究和开发。该平台由一个含有不同细胞因子的培养基分配器、一个允许静态或动态交换培养基的培养单元和一个分别用于泵送和过程控制的中央单元组成。该平台首先用于具有高培养基交换率的组织培养皿的细胞培养，由于营养物质的吸收增强和代谢物的清除增强，细胞生长增加。然后，该平台用于将人诱导多能干细胞 (hiPSC) 自动分化为涉及六种培养基的心肌细胞。结果，经过两周的自动处理，可以获得功能性心肌细胞，显示大范围内有规律的跳动。描述了一些细节，以促进该平台在其他类型的干细胞分化或基于细胞的检测中的应用。