INTRODUCTION Knowing the risk of potential sporadic Creutzfeldt-Jakob disease (sCJD) instrument-contamination is essential in hospitals. We examined the relevance of the p-Tau/Tau ratio to exclude a probable case of sCJD in clinical practice, and we established an alert system to quickly inform health professionals in case of positivity. METHODS This retrospective study was conducted on 143 cerebrospinal fluid samples from patients suspected for sCJD. The distinction between probable cases of sCJD and other patients was based on clinical, paraclinical and biological (14-3-3, Tau, p-Tau, Aβ 1-42) data. From this experience, the health professionals developed an alert system to be implemented upon a suspected case of sCJD. RESULTS A significant decrease in p-Tau/Tau ratio between sCJD and the other diseases was observed (p < 0 .001). The combined Tau test presented a sensitivity higher than 14-3-3 (100% versus 92.3%, p =0 .006) and an equivalent specificity (90% versus 96.1%). The time required for obtaining results was higher for 14-3-3 due to the centralization of investigations in some laboratories (3 weeks versus 2 h). In the presence of these elements, the triggering of the alert system was based on the p-Tau/Tau ratio. This system involves sending an automatic mail to the hospital department involved in the patient's care and the hospital hygiene team, which oversees the application of the procedures. CONCLUSION The p-Tau/Tau concentrations present the desired criteria for use in current medical practice to fight against iatrogenic transmission. The alert system confirms a probable case of sCJD instantly to health professionals. Hygiene and sterilization measures can be applied immediately.

中文翻译：

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一种基于 p-Tau/Tau 比率的自动警报系统，可将散发性克雅氏病的疑似病例快速通知卫生专业人员。

介绍 了解潜在散发性克雅氏病 (sCJD) 器械污染的风险在医院中至关重要。我们检查了 p-Tau/Tau 比率的相关性，以排除临床实践中可能的 sCJD 病例，并且我们建立了一个警报系统，以在阳性的情况下快速通知卫生专业人员。方法 本回顾性研究对 143 份疑似 sCJD 患者的脑脊液样本进行了研究。sCJD 可能病例与其他患者的区别基于临床、临床和生物学（14-3-3、Tau、p-Tau、Aβ 1-42）数据。根据这一经验，卫生专业人员开发了一个警报系统，用于对疑似 sCJD 病例实施。结果 观察到 sCJD 和其他疾病之间的 p-Tau/Tau 比值显着降低 (p < 0 .001)。联合 Tau 检验的灵敏度高于 14-3-3（100% 对 92.3%，p = 0 .006）和等效的特异性（90% 对 96.1%）。由于一些实验室的集中调查（3 周对 2 小时），14-3-3 获得结果所需的时间更长。在存在这些元素的情况下，警报系统的触发基于 p-Tau/Tau 比率。该系统包括向参与患者护理的医院部门和负责监督程序应用的医院卫生团队发送自动邮件。结论 p-Tau/Tau 浓度代表了当前医疗实践中对抗医源性传播所需的标准。警报系统立即向卫生专业人员确认 sCJD 的可能病例。