Soil salinity is a major abiotic stress that affects crop productivity. Garden asparagus (Asparagus officinalis L.) is a perennial plant with some salt tolerance. However, little is known about its response mechanism to salinity stress. In this study, we conducted transcriptome analysis in the leaves of A. officinalis seedlings treated with NaCl solution for 0 h, 1 h, 24 h, and 72 h using the Illumina HiSeq™ 2500 sequencing platform. Compared with the control (0 h), 1027, 3387, and 3358 differentially expressed genes (DEGs) were identified at 1 h, 24 h, and 72 h, respectively. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed that these DEGs were highly enriched in carbon metabolism, ion transport, and reactive oxygen species (ROS) metabolism functional categories, suggesting their key positions in salinity stress responses. Moreover, k-means clustering categorized these DEGs into five kinds of expression patterns at four time points. Of these, DEGs involved in carbon metabolism, ion transport, and ROS metabolism and the groups which they belonged to were identified, which demonstrated their time-dependent response mechanisms. Overall, the transcriptome analysis shed light on the salinity stress response mechanisms in A. officinalis and provided a basis for future studies on salt-tolerance molecular improvement.

中文翻译：

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转录组学和生理学分析揭示了花园芦笋 (Asparagus officinalis L.) 对盐度胁迫的动态响应

土壤盐分是影响作物生产力的主要非生物胁迫。花园芦笋（Asparagus officinalis L.）是多年生植物，具有一定的耐盐性。然而，对其对盐胁迫的反应机制知之甚少。在本研究中，我们使用 Illumina HiSeq™ 2500 测序平台对用 NaCl 溶液处理 0 小时、1 小时、24 小时和 72 小时的厚朴幼苗的叶子进行了转录组分析。与对照 (0 h) 相比，分别在 1 h、24 h 和 72 h 鉴定了 1027、3387 和 3358 个差异表达基因 (DEG)。基因本体论 (GO) 和京都基因和基因组百科全书 (KEGG) 分析表明，这些 DEG 在碳代谢、离子传输和活性氧 (ROS) 代谢功能类别中高度富集，表明它们在盐胁迫反应中的关键位置。此外，k-means 聚类在四个时间点将这些 DEG 分为五种表达模式。其中，确定了参与碳代谢、离子转运和 ROS 代谢的 DEGs 及其所属的组，证明了它们的时间依赖性响应机制。总体而言，转录组分析揭示了厚朴的盐度应激反应机制，并为未来耐盐分子改进的研究提供了基础。这证明了它们的时间依赖性响应机制。总体而言，转录组分析揭示了厚朴的盐度应激反应机制，并为未来耐盐分子改进的研究提供了基础。这证明了它们的时间依赖性响应机制。总体而言，转录组分析揭示了厚朴的盐度应激反应机制，并为未来耐盐分子改进的研究提供了基础。