Reactive oxygen species (ROS) are important regulators of intracellular signaling pathways in health and disease. It is implicated that ROS may play critical roles in pathogenesis of a number of kidney diseases including diabetic nephropathy. However, due to the lack of tools for in vivo detection of redox status, our knowledge of redox dynamics is still fragmentary. In this study, we present novel zebrafish UAS transgenic lines expressing mitochondrial and cytoplasmic targeted redox fluorescent biosensors, Grx1-roGFP2 and mitoGrx1-roGFP2. As the zebrafish is an ideal animal model for intravital imaging, these transgenic zebrafish provide useful tools to analyze renal redox dynamics in vivo.

中文翻译：

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新型转基因品系可体内分析肾脏谷胱甘肽氧化还原电位。

活性氧（ROS）是健康和疾病中细胞内信号通路的重要调节剂。暗示ROS可能在包括糖尿病性肾病在内的许多肾脏疾病的发病机理中起关键作用。但是，由于缺乏用于体内检测氧化还原状态的工具，我们对氧化还原动力学的认识仍然是零碎的。在这项研究中，我们目前表达线粒体和细胞质靶向氧化还原荧光生物传感器，Grx1-roGFP2和mitoGrx1-roGFP2的新型斑马鱼UAS转基因株系。由于斑马鱼是用于活体内成像的理想动物模型，因此这些转基因斑马鱼提供了有用的工具来分析体内的肾脏氧化还原动力学。