Granulocyte/macrophage colony-stimulating factor (GM-CSF) and macrophage CSF (M-CSF) modulate differentiation and immune functions of macrophages (MΦ). Our aim was to evaluate how different MΦ differentiation conditions influence the MΦ response to virus infection. To address this, we differentiated bone marrow-derived MΦ in either GM-CSF or M-CSF and measured the cytokine responses to two different strains of lymphocytic choriomeningitis virus (LCMV) (clone 13; Cl13 or Armstrong; ARM). GM-CSF MΦ infected with either LCMV-ARM or -Cl13 produced more IL-6 than M-CSF MΦ, whereas M-CSF MΦ generated more IL-10 than GM-CSF MΦ. Interestingly, in M-CSF MΦ, LCMV-ARM induced more IL-10 production than Cl13. However, we could not detect any IL-12p70 or IL-23 after infection from either cell types. We also observed that GM-CSF MΦ was more efficient than M-CSF MΦ in supporting antigen-specific CD8⁺ T cell proliferation. Taken together, our data demonstrate that GM-CSF and M-CSF MΦ differ in how they respond to viral infection by their production of different cytokines, and their support for CD8⁺ T cell proliferation.

中文翻译：

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粒细胞/巨噬细胞集落刺激因子衍生的巨噬细胞表现出明显的对淋巴细胞性脉络膜脑膜炎病毒感染的早期免疫反应。

粒细胞/巨噬细胞集落刺激因子（GM-CSF）和巨噬细胞CSF（M-CSF）调节巨噬细胞（MΦ）的分化和免疫功能。我们的目的是评估不同的MΦ分化条件如何影响MΦ对病毒感染的反应。为了解决这个问题，我们在GM-CSF或M-CSF中区分了骨髓来源的MΦ，并测量了对两种不同株的淋巴细胞性脉络膜脑膜炎病毒（LCMV）（克隆13； Cl13或Armstrong； ARM）的细胞因子反应。用LCMV-ARM或-Cl13感染的GM-CSFMΦ比M-CSFMΦ产生更多的IL-6，而M-CSFMΦ比GM-CSFMΦ产生更多的IL-10。有趣的是，在M-CSFMΦ中，LCMV-ARM比Cl13诱导更多的IL-10产生。但是，在任何一种细胞类型感染后，我们都无法检测到任何IL-12p70或IL-23。⁺ T细胞增殖。综上所述，我们的数据表明GM-CSF和M-CSFMΦ在产生病毒因子和对CD8 ⁺ T细胞增殖的支持方面对病毒感染的反应方式不同。