The aim of this study was to detect and characterize isolates of methicillin-/oxacillin-resistant Staphylococcus aureus (MRSA) carrying gene mecC (MRSA/mecC) and occurring in the Czech Republic within the period from 2002 to 2017. Altogether, 18 from 3,472 isolates of MRSA were mecC positive (0.52%). The first detection of MRSA/mecC in the Czech Republic is dated to 2004. MRSA/mecC isolates were susceptible to almost all tested antibiotics with few exceptions. Resistances to erythromycin (n = 2), clindamycin (n = 1), trimethoprim-sulfamethoxazole (n = 1), and rifampicin (n = 1) were found in the collection. Multilocus sequence typing and spa typing revealed a genetic heterogeneity of MRSA/mecC strains: three CCs (130, 425, and 2361), five STs (1245, 130, 2361, 425, and a new ST5480), and eight spa types (t843, t978, t1048, t1535, t1736, t6104, t8842, and t17153), which were detected in the study, with the highest prevalence of CC130/t843 lineage (n = 8, 44%). Except for two strains, none from 18 examined isolates harbored genes encoding any of S. aureus toxins: enterotoxins a–u, exfoliative toxins A, B, and D, toxic shock syndrome toxin-1, and the Panton-Valentine leukocidin.

中文翻译：

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2002-2017年捷克耐甲氧西林/苯唑西林基因mecC金黄色葡萄球菌的表型和基因型特征。

本研究的目的是检测和表征2002 年至 2017 年期间在捷克共和国发生的带有mecC基因(MRSA/ mecC )的耐甲氧西林/苯唑西林金黄色葡萄球菌(MRSA)分离株。总共 18株来自 3,472 MRSA 分离株为mecC阳性 (0.52%)。捷克共和国首次检测到 MRSA/ mecC是在 2004 年。MRSA / mecC分离株几乎对所有测试的抗生素都敏感，只有少数例外。对红霉素 ( n  = 2)、克林霉素 ( n  = 1)、甲氧苄啶-磺胺甲恶唑 ( n  = 1) 和利福平 ( n = 1) 在集合中找到。多位点序列分型和spa分型揭示了 MRSA/ mecC菌株的遗传异质性：三个 CC（130、425和 2361）、五个 ST（1245、130、2361、425 和一个新的 ST5480）和八个spa类型（t843 、t978、t1048、t1535、t1736、t6104、t8842 和 t17153)，在研究中检测到，CC130/t843 谱系的患病率最高 ( n  = 8, 44%)。除了两个菌株外，18 个检测的分离株中没有一个含有编码任何金黄色葡萄球菌毒素的基因：肠毒素 a-u、剥脱性毒素 A、B 和 D、中毒性休克综合征毒素 1 和 Panton-Valentine leukocidin。