In this paper, N, Fe-codoped carbon dots (N, Fe-CDs) were synthesized from β-cyclodextrin, ethylenediamine, and ferric chloride for the first time using a convenient one-step hydrothermal method. The obtained N, Fe-CDs were characterized by various methods including transmission electron microscopy, X-ray photoelectron spectroscopy, and Fourier-transform infrared spectroscopy. The N, Fe-CDs exhibited better catalytic activity than horseradish peroxidase (HRP) and caused an evident color change for 3,3,5,5-tetramethylbenzidine in the presence of H₂O₂. Kinetic experiments show that the apparent K_m value for the N, Fe-CDs with TMB (0.40 mM) or H₂O₂ (0.35 mM) as the substrate was lower than that of HRP (0.43 and 3.70 mM), suggesting that the N, Fe-CDs have a much higher affinity for TMB and H₂O₂ than HRP. The K_m/V_max value for the N, Fe-CDs ( for H₂O₂) is significantly lower than that for HRP (), suggesting that the N, Fe-CDs have a stronger catalytic efficiency for H₂O₂ than HRP. Furthermore, a highly efficient and sensitive colorimetric detection method for glucose was developed using the N, Fe-CDs as mimic peroxidase to detect the hydrogen peroxide generated by the oxidation of glucose by glucose oxidase. The limit of detection for H₂O₂ and glucose was found to be 0.52 and 3.0 μM, respectively. The obtained N, Fe-codoped carbon dots, which possess simulated peroxidase activity, can potentially be used in the field of biotechnology.

中文翻译：

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一步法热合成N，Fe掺杂的碳点作为模拟过氧化物酶及其在过氧化氢和葡萄糖检测中的应用

在本文中，首次使用方便的一步水热方法，由β-环糊精，乙二胺和氯化铁合成了N，Fe掺杂的碳点（N，Fe-CDs）。通过各种方法对获得的N，Fe-CD进行表征，包括透射电子显微镜，X射线光电子能谱和傅里叶变换红外光谱。的N，Fe基的CD显示出比辣根过氧化物酶（HRP）和引起了明显的颜色变化更好的催化活性为3,3 ， 5,5 -在H存在四甲基联苯胺₂ Ò ₂。动力学实验表明，具有TMB（0.40 mM）或H ₂ O的N，Fe-CD的表观K _m值₂（0.35 mM）作为底物低于HRP（0.43和3.70 mM），这表明N，Fe-CD与HRP相比对TMB和H ₂ O ₂具有更高的亲和力。N，Fe-CDs的K _m / V_最大值（H ₂ O ₂）显着低于HRP（H ₂ O ₂）），表明N，Fe-CDs对H ₂ O _2的催化效率比HRP强。此外，开发了一种高效，灵敏的葡萄糖比色检测方法，使用N，Fe-CDs作为模拟过氧化物酶来检测葡萄糖被葡萄糖氧化酶氧化产生的过氧化氢。发现H ₂ O ₂和葡萄糖的检出限分别为0.52和 3.0μM。具有模拟的过氧化物酶活性的所获得的N，Fe共掺杂的碳点可以潜在地用于生物技术领域。